| 广州地区无偿献血人群HHV-8 DNA检测及部分基因的序列分析 |
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| 引用本文： | 付涌水,周豪杰,管洪宇,聂咏梅,汪传喜,江朝富.广州地区无偿献血人群HHV-8 DNA检测及部分基因的序列分析[J].热带医学杂志,2006,6(4):376-378. |
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| 作者姓名： | 付涌水 周豪杰 管洪宇 聂咏梅 汪传喜 江朝富 |
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| 作者单位： | 广州血液中心,广州,510095 |
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| 基金项目： | 2003年广州市医药卫生重点课题(No.2003-ZDi-09)。 |
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| 摘 要： | 目的了解广州地区无偿献血人群人类疱疹病毒8型(HHV-8)的感染情况。方法从全血标本中提取基因组DNA,用巢式聚合酶链反应检测HHV-8DNA开放读码框26基因(ORF26),对其中一阳性PCR产物用双脱氧链终止法进行核苷酸序列测定,测序结果用DNAstar软件分析。结果239例献血者标本中有2例(0.84%)扩增出一条约233bpDNA电泳带。经序列分析证实为HHV-8DNA片段。结论广州地区无偿献血者存在HHV-8感染,但感染率较低。
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| 关 键 词： | 无偿献血者 人类疱疹病毒8 巢式聚合酶链反应 |
| 文章编号： | 1672-3619（2006）04-0376-03 |
| 收稿时间： | 2005-08-24 |
| 修稿时间： | 2005-10-09 |
Detection and Sequence Analysis of Human Herpesvirus 8 DNA in Unpaid Blood Donors in Guangzhou |
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| FU Yong-shui,ZHOU Hao-jie,GUAN Hong-yu,NIE Yong-mei,WANG Chuan-xi,JIANG Chao-fu.Detection and Sequence Analysis of Human Herpesvirus 8 DNA in Unpaid Blood Donors in Guangzhou[J].Journal Of Tropical Medicine,2006,6(4):376-378. |
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| Authors: | FU Yong-shui ZHOU Hao-jie GUAN Hong-yu NIE Yong-mei WANG Chuan-xi JIANG Chao-fu |
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| Abstract: | Objective To investigate the prevalence of HHV-8 infection in unpaid blood donors in Guangzhou. Method Genomic DNA was extracted from 239 samples of whole blood and nested PCR was used to detect HHV-8 ORF26 gene. DNA sequence of positive PCR product was determined by sequencing and analyzed by using DNAstar software. Result A DNA band of approximately 233 base pairs was amplified from 2 samples. Sequence analysis confirmed that the amplified fragment was HHV-8 ORF26 fragment. The positive detection rate of HHV-8 DNA in these 239 samples was 0.84%. Conclusion The prevalence rate of HHV-8 in unpaid blood donors in Guangzhou was quite low. |
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| Keywords: | unpaid blood bonors human herpesvirus 8 nested PCR |
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