强直性脊柱炎患者TNF-α与MMP-3的相关性

目的研究强直性脊柱炎患者中MMP-3与TNF-α间的作用机制。方法在体外分别分离出5例As患者的外周血单个核细胞（PBMC）和6例As患者的膝关节液中的单个核细胞（SFMC），利用不同浓度人重组MMP-3分别在体外诱导PBMC和SFMC，然后应用ELISA技术检测诱导前后TNF-α度变化；同时在分离了7例脊柱关节病（SpA）患者的PBMC后，又进一步分离出它们的贴壁细胞（adherent cells），研究MMP-3是否选择性地作用于单核-巨噬细胞系统还是淋巴细胞系统。结果不同浓度的MMP-3均能诱导AS患者PBMC释放TNF-α并且TNF-α的浓度具有剂量依赖性；MMP-3能够明显诱导贴壁细胞分泌TNF-α；MMP-3对SFMC具有一定程度的诱导作用，但同时还观察到与PBMC不同的是，即使没有MMP-3的诱导作用，SFMC自身也能分泌一定程度的TNF—α。结论AS患者中，MMP-3与TNF-α之间的相互作用可能是参与其发病的重要原因之一，同时MMP-3可能是通过选择性地作用于AS患者的单核-巨噬细胞系统，而非通过淋巴细胞系统来发挥作用。

Correlation between tumor necrosis factor-alpha and matrix metalloproteinase-3 in ankylosing spondylitis patients

Objective To study the mechanism of action between matrix metalloproteinase-3 （ MMP-3 ） and tumor necrosis factor-alpha （TNF-α） in ankylosing spondylitis （AS） patients. Methods After peripheral blood mononuclear cells （PBMC） were isolated from 5 AS patients and synovial fluid mononuclear cells （SFMC） were isolated from 6 AS patients with synovial fluids in their knee joints, the PBMC and SFMC were stimulated in vitro with human recombinant MMP-3 and changes in TNF-α expression were detected by enzyme linked immunosorbent assay（ELISA）. （Adherent cells were also isolated from PBMC of 7 patients with spondyloarthropathy （SpA） using （human recombinant MMP-3 ） stimulated adherent cells in vitro and the concentration of TNF-α was determined by ELISA）. Results Different concentrations of human recombinant MMP-3 induced release of TNF-α by stimulating PBMC in vitro in a dose-dependent manner. The SFMC of AS patients could automatically produce TNF-α even though it was not stimulated by human recombinant MMP-3. Conclusion Interaction between MMP-3 and TNF-α may play an important role in the pathogenesis of AS. MMP-3 may also selectively stimulate monoeytes or maerophages rather than lymphocytes.