曲伐沙星经抑制NF-κB的激活调控LPS诱导的炎症反应

目的探讨曲伐沙星抗炎作用的分子机制。方法将分离的单核细胞预先用TVF孵育2h，随后用LPS刺激，ELISA检测TNF-α和IL-6的诱生情况，并用RT-PCR检测其mRNA的表达；提取核蛋白，Westem blot分析NF—κB激活情况。结果TVF能以剂量依赖方式抑制单核细胞产生TNF-α和IL-6，并抑制其mRNA的表达；同时TVF能抑制NF—κB的激活。结论TVF能抑制单核细胞产生TNF-α和IL-6，这可能是其抗炎症作用的方式之一，该效应可能与其抑制NF—κB的激活有关。

Trovafloxacin Regulates LPS-induced Inflammatory Response by Inhibits NF-κB Activation

Objective To investigate the anti-inflammatory activity of trovafloxaein （TVF）. Methods Isolated monocytes were preincubated with TVF for 2h, and then stimulated by LPS. Induction of TNF-α and IL-6 was detected by ELISA, and their mRNA levels were assessed by RT-PCR; Nucleoprotein were extracted and activation of NF-κB was detected by Western blot. Results TVF inhibited monocytes production of TNF-α and IL-6 in a dose-dependent manner, and inhibited their mRNA expression, NF-κB was also inhibited by TVF either. Conclusion The anti-inflammatory activity of TVF occurrs by inhibit TNF-α and H.-6 production, this effect is probablely mediated by inhibited NF-κB activation.