血小板活化因子在颈髓损伤后线粒体功能损伤中的作用

目的和方法；采用Ａｌｌｅｎ打击法造成Ｃ６．７损伤，鞘内注射血小板活化因子及静脉注射ＰＡＦ受体拮抗剂ＮＢ５２０２１，观察其对颈颈髓损伤后脊髓组织ＰＡＦ含量，颈髓线粒体ＡＴＰ酶活性，线粒体呼吸功能的影响，结果；颈髓损伤后颈髓组织ＰＡＦ含量明显增加，蛛网膜下腔注射ＰＡＦ可使伤后ＰＡＦ含量增加更为显著；ＰＡＦ能够抑制Ｃａ＾２＋－ＮＭｇ＾２＋－ＡＴＰ酶，Ｎａ＾＋－Ｋ＾＋－ＡＴ「Ｐ酶活性，明显降低线粒体耗年头

Effects of platelet activating factor and its receptor on mitochondrial function damage after cervical cord injury

AIM and METHODS:C 6,7 spinal cord injury models were performed in cats by Allen's method. Platelet activating factor (PAF) and PAF receptor antagonist BN 52021 were administered by intrathecal or intravenous injection respectively. Their effects on PAF level, mitochondrial ATPase activity, mitochondrial function in cervical cord were observed after cervical cord injury. RESULTS:PAF levels in cervical cord tissue significantly increased following trauma. PAF levels were evidently elevated by arachnoid space injection of PAF. PAF receptor antagonist BN 52021 could inhibit the increase of PAF levels in the cervical cord tissue following trauma; PAF could inhibit mitochondrial Na -K -ATPase, Ca 2 -Mg 2 -ATPase activities and evidently decrease mitochondrial oxygen uptake rate (OUR), respiratory control rate (RCR), P/O ratio and oxidative phosphorylation rate (OPR); PAF receptor antagonist BN 52021 could sustain the relative stability of Na -K -ATPase, Ca 2 -Mg 2 -ATPase activity and improve mitochondrial respiratory function. CONCLUSION:PAF and its receptor play an important role in the pathophysiology process of mitochondrial function damage after cervical cord injury. PAF antagonist can effectively protect mitochondrial function after trauma.