一氧化氮、内皮素和循环内皮细胞在支气管哮喘中的作用研究

目的：探讨一氧化氮、内皮素和循环内皮细胞在支气管哮喘中的作用。方法：哮喘患儿６１例，４０名健康儿童为对照，测定其血浆ＮＯ－２／ＮＯ－３、ＥＴ、ｃＧＭＰ和循环内皮细胞（ＣＥＣ）水平，用过敏性哮喘豚鼠进行验证。结果：哮喘患儿血浆ＮＯ－２／ＮＯ－３、ｃＧＭＰ和ＣＥＣ水平均明显升高（Ｐ＜０．０５～００１），其中重度哮喘患儿血浆ＮＯ－２／ＮＯ－３和ＣＥＣ水平较轻中度哮喘患儿更高（Ｐ均＜００１）；重度哮喘患儿血浆ＥＴ水平明显升高（Ｐ＜００１）；哮喘患儿血浆ＮＯ－２／ＮＯ－３与ｃＧＭＰ和ＣＥＣ水平呈显著正相关（Ｐ＜００５～００１）。过敏性哮喘豚鼠ＢＡＬＦ中ＮＯ－２／ＮＯ－３、ＥＴ及ｃＧＭＰ水平均明显升高（Ｐ均＜００１）；血浆中ｃＧＭＰ和ＣＥＣ水平亦升高（Ｐ均＜００５）；糖皮质激素可降低ＮＯ－２／ＮＯ－３、ＥＴ、ｃＧＭＰ和ＣＥＣ水平。结论：哮喘时ＮＯ－２／ＮＯ－３、ＥＴ和ＣＥＣ产生过多，它们在哮喘发病机制中占有重要地位，而且血浆ＥＴ和ＣＥＣ水平可能与哮喘病情相关。

Roles of NO, ET and CEC in the pathogenesis of asthma

AIM:To investigate the roles of NO,endothelin(ET) and circulating endothelial cells (CEC) in the pathogenesis of asthma. METHODS:Plasma nitrite and nitrate (NO - 2/NO - 3), ET, cGMP and CEC levels in 61 asthmatic children and 40 matched normal children were examined. In addition, the plasma and bronchoalveolar lavege fluid (BALF) NO - 2/NO - 3, ET, cGMP and CEC levels in guinea-pigs with allergic asthma were determined.RESULTS:Asthmatic children had increased amounts of NO - 2/NO - 3, cGMP and CEC compared with normal control subjects ( P<0.05, P<0.01 ).Among them, 15 severe asthmatic children had significantly higher levels of ET than normal subjects ( P<0.01 ). Elevations of NO - 2/NO - 3 were correlated with cGMP and CEC( P <0.05, P< 0.01). Guinea-pigs with allergic asthma had increased amounts of NO - 2/NO - 3,ET and cGMP in BALF than normal control subjects ( P<0.01 ),so did cGMP and CEC in plasms( P <0.05). Glucocorticoid may have reduced NO - 2/NO - 3, ET, cGMP and CEC contents and even have therapeutic effects.CONCLUSIONS:There were increased production of NO - 2/NO - 3, ET, and CEC in asthma, they may play an important roles in the pathogenesis of asthma. ET and CEC may be useful in forecasting the severity of illness.