SOD对脑缺血-再灌注损伤大鼠谷氨酸转运体功能的影响

目的：探讨超氧化物歧化酶（ＳＯＤ）对脑缺血再灌注损伤（Ｉ－Ｒ）大鼠皮层、海马、太体氨酸转运体功能的影响。方法：采用大鼠３个血管夹闭、松夹复制脑Ｉ－Ｒ损伤模型。利用脑组织突触膜顾粒对「＾３Ｈ」－Ｌ－谷氨酸摄入量的测定及分光光度法观察ＳＯＤ对皮层海马纹状体谷氨酸转运体功能、丙二醛（ＭＤＡ）含量及ＳＯＤ活性的影响。结果：Ｉ－Ｒ组谷氨酸转运体的功能及ＳＯＤ活性明显低于对照组，ＭＤＡ含量明显高于对照组。ＳＯ

Influence of SOD on the function of glutamate transporter in ra ts with injury of cerebral ischemia-reperfusion

AIM:To investigate the effect of superoxide dismutase(SOD) on the function of glutamate transporter in rats with injury of cerebral ischemia-reperfusion.METHODS:A model of ischemia-reperfusion injury(I-R) was performed by clipping three arteries and then by releasing them to reperfuse blood into ischemic brain in rats. Glutamate transporter functions were studied by means of assay of L-glutamate uptake in synaptosomes, and malondialdehyde (MDA) contents and SOD activities in cerebral contex, hippocampus ,striatum by spectrophotometry.RESULTS:Glutamate transporter function and SOD activity in I-R group significantly decreased ( P <0 05, P< 0 01);content of MDA significantly increased( P <0 05, P< 0 01) compared with control group. Glutamate transporter function and SOD activity in SOD group significantly increased ( P <0 05, P< 0 01);MDA content significantly decreased( P <0 05, P< 0 01) compared with I-R group.CONCLUSION:SOD can improve glutamate transporter function of I-R rats. The mechanism might be related to eliminate free radicals.