动脉内皮损伤后平滑肌细胞维甲酸受体的表达及维甲酸的作用

目的 研究动脉内皮损伤后ＶＳＭＣ中ＲＡＲ表达的规律及其与内膜增生的关系，方法 放射配基法测量用维甲酸与未用药两组内皮损伤后不同时期的受体最大结合容量和平衡解离常数抽时用形态学方法测量血管内膜面积。结果 动脉内皮损伤后早期ＶＳＭＣ中ＲＡＲ数量显著低于正常（Ｐ〈０．０１），于第４周恢复正常，同时血管膜面积增加，用维甲酸后，ＲＡＲ显著增加，于术后２周即接近正常，血管内膜面积显著少于未用药组，结论 动脉损

Retinoic acid receptor expression in arterial smooth muscle cells after endothelial injury and effects of all-trans retinoic acid

AIM: To investigate expression of retinoic acid receptor (RAR) in smooth muscle cells after endothelial injury and its relation to intimal hyperplasia. METHODS:Rabbits were divided into two groups:all-trans retinoic acid (RA) treated group and non-drug group, arteries were harvested 24h, 1, 2 and 4 weeks after injury. Receptor Bmax and K D were calculated by the use of -RA radioligand binding method, neointimal area(IA) were measured morphologically.RESULTS:Compared with normal arteries, number of RAR in smooth muscle cells reduced significantly ( P<0.01 ) in the first two weeks after injury, and recovered to normal in the 4th week, at the meantime, IA increased significantly. In RA treated group, RAR number increased significantly when compared with non-drug group, and were about to normal level 2 weeks after injury. IA decreased at the same time. CONCLUSION:RAR down regulation existed in the early stage after endothelial injury, RA upregulated RAR expression, RAR might play an important role in intimal hyperplasia after artery injury.