大鼠主动脉内膜球囊剥脱后再生内皮细胞表型改变的研究

目的 探索大鼠主动脉内膜球囊剥脱后，内皮修复过程中再生内皮细胞结构和功能的改变，方法 大鼠主动脉内膜球囊剥脱２周和６周对内膜进行形态学观察，同时测定血管反应性，亚硝酸盐量和ＮＯＳ的活性，结果：剥脱２周和６周后，再生内皮细胞不仅结构与正常内皮细胞不同，而且血管再生内皮依赖性舒张反应９减弱，ＮＯ的释放减少，血管组织ｉＮＯＳ和ｃＮＯＳ活性均比正常降低，剥脱６周后的ｉＮＯＳ比剥脱２周时有所增加，但ｃＮＯＳ

Studyb on the changes of structure and function in regenerated endothelial cells after the denudation of endothelium in rat aortic artery

AIM: To investigate the changes of structure and function in regenerated endothelial cells (REC) after the denudation of endothelium in rat aortic artery.METHODS:After the denudation of endothelium in rat artery by balloon, REC structure was revealed by H E staining, the reactivity of endothelial dependent relaxation (EDR) in vessels, release of endothelial nitric oxide(NO) and vascular nitric oxide synthase (NOS) were investigated.RESULTS: REC structures were different from normal endothelial cell; EDR in vessels after denudation for 2 weeks and 6weeks decreased, and release of endothelial nitric oxide(NO) decreased companied with inhibited vascular inducible nitric oxide synthase(iNOS) and constructional nitric oxide synthase(cNOS).CONCLUSION:Phenotypic shift of REC during neointima formation after endothelial removal by balloon-injury, and dysfunction of REC might enhance neointimal impairment during retenosis formation after angioplasty.