血管内皮生长因子及其基因在自发性高血压大鼠心肌中的表达

目的：血管内皮生长因子（ＶＥＧＦ）是新近确定的一种特异作用于血管内皮细胞的活性肽。最近发现正常心肌细胞有ＶＥＧＦ及其基因表达，但对高血压肥大心脏心肌ＶＥＧＦ及其基因表达的变化尚不清楚。方法：本实验采用免疫组化和分子杂交方法，对自发性设备夸大鼠（ＳＨＲ）肥大于心脏心肌ＶＥＧＦ及其基因表达进行研究。结果：免疫组化结果表明：ＳＨＲ心肌细胞浆内特异性ＶＥＧＦ染色颗粒明显多于ＷＫＹ对照大鼠。Ｎｏｔｈｅｒｎ分

Expression of vascular endothelial growth factor and its gene in myocardium of spontaneously hypertensive rats

AIM: Vascular endothelial growth factor(VEGF), a growth and permeability factor specific for endothelial cell, has been identified in myocardium of the normal adult heart, but the change of VEGF in myocardium of hypertensive hypertrophic heart was unclear. METHODS:We therefor studied the expression of VEGF and its gene in myocardium of spontaneously hypertensive rats(SHR) by immunohistochemistry assay and Northen bolt. The results were analyzed by scanning densitometry.RESULTS:Compared with control Wistar Kyoto (WKY) rats, the left ventricle weight/body weight of SHR increased by 61%( P<0.01 ). The intracellular VEGF immunoactivity was present in myocardium of WKY rats and SHR, but the immunoactivity of SHR was 91% stronger than that of WKY rats. The results of Northern bolt showed that the expression of VEGF mRNA of SHR increased by 67% than that of WKY by scanning densitometry. CONCLUSION:Although little is known about the role of expression upregulation of VEGF and its gene in myocardium of SHR, it seems to respond to vascular proliferation of hypertrophic heart.