Correlation of the HPV detection,protein expression and DNA content in cutaneous pre-invasive and invasive carcinoma among Croatian patients |
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Authors: | Kristina Meljanac Salopek Stanko Jukić Damir Babić |
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Affiliation: | Laboratory for Molecular Pathology, Institute of Pathology, University of Zagreb School of Medicine, ?alata 10, 10 000 Zagreb, Croatia |
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Abstract: | Development of cutaneous carcinomas has been associated with HPV infection. There have been various reports on p16, p53 and pRb expression in cutaneous carcinomas and on its linkage to HPV status. Association of protein expression and HPV infection with DNA content is not clear. The aim of this study was to determine a possible correlation between HPV type, protein expression and DNA content in both pre-invasive and invasive squamous cell carcinoma, as well as differences between studied groups in these parameters. Sections of formalin fixed paraffin-embedded tumor tissue from 54 cases of Morbus Bowen (preinvasive cutaneous carcinoma) and 41 cases of invasive squamous cell carcinoma of the skin were subjected to HPV genotyping using Lipa (Line imuno probe assay), immunohistochemical staining for p16(INK4A), p53, pRb and prepared for flow cytometry DNA content analysis. Obtained data were analyzed in SPSS using Chi square test. Only p16 expression showed statistically significant differences in studied groups. Statistically significant correlations were found only in MB between parameters HPV-p53, p53-pRb and p53-p16. Our results suggest different virus-induced pathobiology pathways for different cutaneous carcinoma groups. |
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Keywords: | HPV p16(INK4A) p53 Flow cytometry Squamous cell carcinoma |
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