中药五倍子对不同根面龋损提取非胶原蛋白后再矿化的影响

目的体外观察人工形成的牛牙根面龋提取非胶原蛋白前后,中药五倍子对人工龋再矿化的影响。方法体外37℃恒温箱中,两种脱矿溶液中用牛牙模拟人牙形成不同形态根面龋模型(酸蚀性病损和表层下脱矿)。每组随机选择两个样本用偏光显微镜(PLM)观察表面形态;用盐溶液提取根面龋暴露区的可溶性牙本质磷蛋白(S-DPP),在恒温箱中用五倍子多酚化合物(GCE)对提取非胶原蛋白前后的根面龋同时进行再矿化循环7d,用激光共聚焦扫描显微镜(LSCM)观察样本并分析平均荧光量。结果 PLM观察显示表层下脱矿形成了较完好的表层,而酸蚀性病损则无表层形成。LSCM分析结果显示在酸蚀性病损形态下提取S-DPP后GCE处理荧光强度明显下降(P<0.05),而在表层下脱矿形态下提取S-DPP前后GCE处理荧光强度无明显差异(P>0.05)。结论在酸蚀性病损条件下提取S-DDP后GCE可能有更高的再矿化效果。

Re-mineralization ability of Galla chinensis extracts on root carious lesions with or without non-collagen proteins

Objective To evaluate the re-mineralization ability of Galla Chinensis extracts(GCE) on two artificial carious lesions in bovine root de-mineralized in vitro.Methods Fourteen bovine root blocks were divided into two parts from buccal to lingual direction.The mesial blocks were treated with a demineralization solution and the distant blocks were treated with another demineralization solution.Two specimens from each group were selected randomly and examined with polarization microscope(PLM).After all blocks were demineralized,half surface of the demineralized zone was covered and the another half was treated with 0.5% NaCl to extract soluble dentin phosphate protein(S-DPP).Then all specimens were submitted to pH-cycling for one week.In the first four days,all specimens were treated with GCE for 21 h and with demineralization solution for 3 h.In the remaining three days,all specimens were treated with GCE.The re-mineralization ability of GCE on the specimens was evaluated by laser scanning confocal microscope(LSCM).Results There existed intact surface layers on subsurface lesions but no surface layers were produced on erosive lesions.The re-mineralization ability of GCE on erosive lesions improved significantly with the treatment of 0.5% NaCl solution(P<0.05).But it had no significant effect on subsurface lessions.Conclusion Extraction of S-DPP with 0.5% NaCl can improve the re-mineralization ability of GCE on root caries with erosive lesions.This finding supports the proposition that Galla Chinesis may be a promising anti-caries natural medicine in the future.