高效液相色谱法同时测定强氯滴眼液中2组分的含量

目的:建立以高效液相色谱法同时测定强氯滴眼液中氯霉素和醋酸泼尼松龙含量的方法。方法:色谱柱为HiQsilC18,流动相为甲醇-水-冰醋酸(70∶30∶0.04),检测波长为278nm,流速为0.8ml/min,柱温为30℃,进样量为10μl。结果:氯霉素与醋酸泼尼松龙检测浓度分别在22.36～89.44、25.00～100.00μg/ml范围内线性关系良好;平均加样回收率分别为99.65%(RSD=0.88%)、99.57%(RSD=1.02%)。结论:本方法简便、准确、重现性好,能同时测定强氯滴眼液中2组分的含量。

Contents Determination of 2 Components in Hadro- chlorine Gutta by HPLC

OBJECTIVE:To establish the HPLC method for the simultaneous content determination of chloramphenicol and prednisolone acetum in hadro-chlorine gutta.METHODS:The chromatographic column was HiQ sil C 18 ,the methanol-water-glacial acetic acid(70∶30∶0.04)served as mobile phase,and the detecting wavelength was278nm,the flow rate was0.8ml/min and the column temperature was30℃,the sample size was10μl.RESULTS:A satisfactory linear relationship beˉtween was found when the concentration determination ranges of chloramphenicol and prednisolone acetum at22.36～89.44μg/ml and25.00～100.00μg/ml respectively;The average recoveries for chloramphenicol and prednisolone acetum were99.65%(RSD=0.88%)and99.57%(RSD=1.02)respectively.CONCLUSION:The method was simple,accurate and good in repro-ducibility,which can be used for the simultaneous contents determination of 2 components in hadro-chlorine gutta.