900MHz拟手机电磁辐射对大鼠肝功能和肝细胞Bax及Bel-2蛋白表达的影响

目的观察900MHz拟手机电磁辐射对大鼠肝功能和肝细胞Bax及Bcl-2表达的影响。方法选择sD大鼠20只，按体重均衡的原则随机分为正常组和辐射组，每组10只。正常组大鼠置辐射笼中不接受辐射，辐射组大鼠接受900MHz拟手机电磁辐射连续18d，4h／d。比色法检测大鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）含量，HE染色观察大鼠肝脏组织形态变化，免疫组化法检测大鼠肝细胞Bax及Bcl．2蛋白表达。结果与正常组相比，辐射组大鼠血清ALT显著升高（P〈0．05），AST无明显变化（P〉0．05）；肝细胞核缩小或部分消失以及细胞浆空泡样变明显；肝细胞Bax蛋白表达增强，Bcl-2蛋白表达减弱（P〈0．01）。结论长时间暴露于900MHz拟手机电磁辐射中可造成大鼠肝功能异常、肝组织形态的损伤、肝细胞凋亡。

Influence of Imitated 900 MHz Cellular Electromagnetic Radiation on Liver Function and Bax and Bcl-2 Protein Expressions in Hepatic Cells in Rats

Objective To observe the influence of imitated 900 MHz cellular electromagnetic radiation on liver function and Bax and Bcl-2 protein expressions in hepatic cells in rats. Methods According to weight equilibrium prin- ciple, 20 adult SD rats were randomly divided into normal group （ n = 10） and radiated group （ n = 10）. The rats in nor- mal group was not radiated in the radiation box, while the rats in radiated group were radiated by imitated 900MHz cellu- lar electromagnetic radiation with 4 h/d for 18 d. The contents of alanine transarninase （ALT） and aspartate transarni- nase （AST） in rats＇serum were detected by colorimetric method, the changes of form and function of rats＇hepar function were observed by HE staining, and the bax and Bcl-2 protein expressions in rats~hepatic ceils were detected by immuno- histochemical method. Results Compared with those in normal group, the content of serum ALT in radiated group was significantly increased （P 〈 0.05）, and there were no significantly changes in AST contents （P 〉 0.05） ; liver cell nu- cleus decreased or partially disappeared, and partial vacuolar degeneration was found in cytolymph; there was a higher expression of the Bax protein, and a lower expression of Bcl-2 protein in hepatic ceils （P 〈 0.01 ）. Conclusion The long-time imitated 900 MHz cellular electromagnetic radiation can obviously cause dysfunction of liver, hurt hepatic tissue morphous, and increase the speed of hepatic apoptosis.