Dye coupling in Purkinje cells of organotypic slice cultures |
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Authors: | Meller Karl Krah Kerstin Theiss Carsten |
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Affiliation: | Medizinische Fakult?t, Institut für Anatomie, Abteilung für Cytologie, Ruhr-Universit?t Bochum, Universit?tsstrasse 150, D-44780 Bochum, Germany. |
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Abstract: | Cerebellar slice cultures of newborn rats showed poorly developed dendritic arborization of Purkinje cells, whereas cultures of 10-day-old rats revealed prominent dendritic branching. Gap junctional intercellular communication between Purkinje cells, investigated as dye transfer of microinjected neurobiotin, occurred through dendro-dendritic contacts, with decreased dye spreading in old cell cultures. These results indicate a possible correlation of gap junctional intercellular communication and the development of Purkinje cells. |
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