环状RNA circUGGT2对结直肠癌细胞增殖、迁移和侵袭的影响

目的 探讨环状RNA circUGGT2在结直肠癌（colorectal cancer，CRC）中的表达及其对CRC细胞增殖、迁移和侵袭的影响。 方法 收集粤北人民医院胃肠外科2018年9月至2019年3月45例手术切除的CRC组织及相应癌旁组织标本，采用qRT-PCR检测组织及人正常结肠上皮细胞株NCM460和CRC细胞株HT29、HCT116、SW480、SW620中circUGGT2的表达水平。绘制受试者工作特征（ROC）曲线并计算曲线下面积（AUC）评估circUGGT2的诊断效能。将si-circUGGT2和si-NC分别转染至SW480细胞，采用CCK-8和平板克隆形成实验检测细胞增殖情况，划痕实验和Transwell小室实验检测细胞迁移和侵袭情况。结果 与癌旁组织比较，circUGGT2在CRC组织中高表达（P<0.001），且与患者肿瘤大小、浸润深度和TNM分期有关（均P<0.05）；circUGGT2诊断CRC的AUC为0.702。circUGGT2在4种CRC细胞中的表达水平均高于NCM460细胞（均P<0.001），尤其在SW480细胞中的表达水平最高。与si-NC组比较，沉默circUGGT2后SW480细胞增殖速率和划痕愈合速率降低（均P<0.05），克隆形成数目及迁移、侵袭细胞数明显减少（均P<0.05）。结论 circUGGT2在CRC中高表达，沉默circUGGT2可抑制CRC细胞增殖、迁移和侵袭。circUGGT2可能是CRC诊断和治疗的潜在分子靶点。

Effect of circular RNA circUGGT2 on the proliferation,migration and invasion of colorectal cancer cells

Objective To investigate the expression of circRNA circUGGT2 in colorectal cancer (CRC) and its effect on the proliferation, migration and invasion of CRC cells. Methods A total of 45 surgically resected CRC tissues and paracancerous tissues were collected from the Department of Gastrointestinal Surgery, Yue Bei People's Hospital between September 2018 and March 2019. The expression level of circUGGT2 in human normal colon epithelial cell line NCM460 and CRC cell lines HT29, HCT116, SW480 and SW620 was detected by qRT-PCR. The diagnostic efficacy of circUGGT2 was evaluated by plotting the receiver operating characteristic (ROC) curve and calculating the area under the curve (AUC). The si-circUGGT2 and si-NC were transfected into SW480 cells, respectively. The cell proliferation was detected by CCK-8 assay and clone formation assay. The migration and invasion ability of the cells were detected by scratch experiment and Transwell assay. Results Compared with paracancerous tissues, circUGGT2 was highly expressed in CRC tissues (P<0.001), which was related to tumor size, infiltration depth and TNM stage of patients (all P<0.05); the AUC of circUGGT2 to diagnose CRC was 0.702. The expression level of circUGGT2 in the 4 CRC cells was higher than that in NCM460 cells (all P<0.001), especially in SW480 cells. Compared with the si-NC group, the proliferation rate and scratch healing rate of SW480 cells decreased after silencing circUGGT2 (all P<0.05), and the number of clone formation, migration and invasion cells were also significantly reduced (all P<0.05). Conclusions circUGGT2 is highly expressed in CRC, and silencing circUGGT2 can inhibit the proliferation, migration and invasion of CRC cells. circUGGT2 is a potential molecular target for diagnosis and treatment of CRC.