金花茶叶醇提物通过调节胆固醇逆向转运关键蛋白的表达抑制动脉粥样硬化

目的:探讨金花茶叶醇提物调节胆固醇逆向转运(RCT)关键蛋白细胞质膜微囊蛋白-1(caveolin-1)、清道夫受体B1(SR-B1)、A1型三磷酸腺苷结合盒转运体(ABCA1)、蛋白激酶A(PKA)的表达而抑制巨噬细胞向泡沫细胞衍变、拮抗动脉粥样硬化(AS)的潜在药理作用机制.方法:乙醇回流提取获得金花茶叶醇提物,制...

Ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves attenuated atherosclerosis via regulating the expressions of key proteins during cholesterol reverse transport

OBJECTIVE To explore the regulation of ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves on the expressions of key proteins during reverse cholesterol transport(RCT),i.e.plasma membrane microcapsule protein-1(caveolin-1),scavenger receptor B1(SR-B1),type A1 adenosine triphosphate binding box transporter(ABCA1)and protein kinase A(PKA),as a potential pharmacological mechanism by which foam cell evolvement from macrophage and atherosclerosis(AS)would be attenuated. METHODS Ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves were purified by ethanol reflux.Ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves-containing rat sera was prepared.The regulations of ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves-containing rat serum on classical macrophage activation,foam cell evolvement after phagocytizing ox-LDL and expressions of caveolin-1,SR-B1,ABCA1,PKA were measured in vitro.The animal model of AS was established by male ApoE^-/- mice with high-fat feeding.The regulatory effects of ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves on AS plaque formation and the expressions of caveolin-1,SR-B1,ABCA1,PKA in murine intima were detected in vivo. RESULTS The drug-contain sera not only significantly inhibited classical macrophage activation,but also prevented macrophages from evolving into foam cells.Also an up-regulation of caveolin-1,SR-B1,ABCA1 and PKA was observed.Ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves demonstrated a marked up-regulation of caveolin-1,SR-B1,ABCA1 and PKA in aortic arch endomembrane of AS mice and attenuated AS plaque formation. CONCLUSION Ethanol extracts from Camellia chrysantha(Hu)Tuyama leaves can prevent macrophages from evolving into foam cells and attenuate AS plaque formation.It may be correlated with an up-regulation of caveolin-1,SR-B1,ABCA1 and PKA as the key proteins during RCT.