血清miRNA-126水平与乙肝肝细胞癌血管生成拟态的关系

 目的 探讨miRNA-126与乙肝肝细胞癌(hepatocellular carcinoma, HCC) 血管生成拟态(vasculogenic mimicry, VM)的关系。方法 收集2016-10至2019-10接受根治性肝切除术的巴塞罗那分期为Ⅰ-Ⅱa期乙肝相关HCC患者183例为研究对象。同期随机抽取健康体检者183例作为对照组。使用PCR检测并比较两组血清中miRNA-126表达水平。对HCC患者进行病理组织切片的HE染色和CD34-PAS双重染色,分析患者VM阳性的影响因素,并构建预测VM的ROC曲线。结果 HCC患者较对照组血清miRNA-126表达降低。HCC患者中VM阳性74例,VM阴性109例。Logistic回归分析显示miRNA-126低表达(P=0.008),肿瘤大小(P=0.001),AFP升高(P=0.000)与发生VM相关。miRNA-126对VM阳性具有较高预测价值,其AUC=0.869(95%CI:0.815-0.924)。结论 miRNA-126低表达与乙肝HCC患者发生VM相关,miRNA-126可作为潜在判断VM的无创检测指标。

Serum miRNA-126 level and vasculogenic mimicry in patients with hepatitis B hepatocellular carcinoma

Objective To investigate the relationship between miRNA-126 and vasculogenic mimicry (VM) in patients with hepatitis B hepatocellular carcinoma (HCC).Methods A total of 183 patients with hepatitis B related HCC in BCLC stage I-IIa who underwent radical hepatectomy were selected as the research objects from October 2016 to October 2019. At the same time, 183 healthy people were randomly selected as the control group. The expression of miRNA-126 in serum was detected by PCR. Then, HE staining and CD34-PAS double staining were used to analyze the influencing factors of VM positive in HCC patients. The ROC curve for predicting VM was constructed.Results The expression of serum miRNA-126 in HCC patients was lower than that in control group. Among HCC patients, 74 cases were VM positive and 109 cases were VM negative. The result of logistic regression analysis showed that low expression of miRNA-126(P=0.008), tumor size (P=0.001) and increased AFP (P=0.000) were associated with VM. miRNA-126 has a high predictive value for VM positive, AUC=0.869(95%CI:0.815-0.924). Conclusions The expression of miRNA-126 can be used as a noninvasive marker of HCC.