MACC1、SPON2在胃癌组织中的表达及其临床意义

目的探讨结肠癌转移相关基因1(metastasis associated in colon cancer 1,MACC1)、脊椎蛋白2(spondin 2,SPON2)在胃癌组织中的表达及其临床意义。方法收集2013年1月至2015年1月于本院行胃癌根治术的106例胃癌患者癌组织、90例癌旁正常胃黏膜组织以及内镜下摘取的83例胃息肉组织。采用免疫组化法检测MACC1、SPON2蛋白表达情况。Cox比例风险回归分析影响胃癌预后的因素。结果MACC1、SPON2蛋白在胃癌组织的阳性表达率均高于胃息肉组织及癌旁正常胃黏膜组织(均P<0.05)。胃癌组织中MACC1、SPON2表达水平均与肿瘤分化程度、TNM分期、浸润程度及淋巴结转移有关(均P<0.05)。MACC1蛋白高表达组和低表达组中位总生存时间分别为23个月和34个月(log-rankχ^(2)=9.324,P=0.002);SPON2蛋白高表达组和低表达组中位总生存时间分别为25个月和34个月(log-rankχ^(2)=4.694,P=0.030)。多因素分析结果显示,MACC1及SPON2蛋白高表达是胃癌患者预后不良的危险因素(均P<0.05)。结论MACC1、SPON2在胃癌组织中高表达,两者高表达均是胃癌患者预后不良的因素。

Expression and clinical significance of MACC1 and SPON2 in gastric cancer

Objective To investigate the expression of metastasis associated with colon cancer 1 (MACC1) and spondin 2 (SPON2) in gastric cancer tissues and its clinical significance. Methods A total of 106 cancer tissues and 90 adjacent normal gastric mucosa tissues of gastric cancer patients who underwent surgical treatment in Shanghai Eighth People's Hospital from January 2013 to January 2015, were collected, and 83 cases of gastric polyps extracted by endoscopy were selected as well. The expression of MACC1 and SPON2 proteins were detected by immunohistochemistry. The prognosis factors of gastric cancer was analyzed by the Cox proportional hazard model. Results The positive expression rates of MACC1 and SPON2 proteins in gastric cancer tissues were higher than those in gastric polyp tissues and normal gastric mucosa tissues (all P<0.05). The expression levels of MACC1 and SPON2 proteins were correlated with tumor differentiation, TNM stage, the degree of infiltration and lymph node metastasis (all P<0.05). The median overall survival  of MACC1 protein high expression group and low expression group were 23 months and 34 months (log-rank χ² =9.324, P=0.002), respectively. The median overall survival of SPON2 protein high expression group and low expression group were 25 months and 34 months (log-rank χ² =4.694, P=0.030), respectively. The multivariable analysis showed that the high expression of MACC1 and SPON2 proteins were the risk factors for poor prognosis of gastric cancer patients (all P<0.05). Conclusions MACC1 and SPON2 are highly expressed in gastic cancer tissues, and both high expressions are the factors of poor prognosis in patients with gastric cancer.