白藜芦醇对人胃癌耐药细胞SGC-7901/5-FU生物学效应的影响及其作用机制

目的 探讨白藜芦醇(resveratrol,RES)对5-氟尿嘧啶(5-fluorouracil,5-FU)耐药胃癌细胞(SGC-7901/5-FU)生物学效应的影响及其作用机制.方法 用不同浓度(0μmol/L、10μmol/L、50μmol/L、200μmol/L、400μmol/L)的RES分别处理SGC-790...

Effect of resveratrol on biological effects of human gastric cancer SGC-7901/5-FU cells and its mechanisms

Objective  To investigate the effect of resveratrol (RES) on the biological effects of SGC-7901/5-FU cells and its mechanisms. Methods The SGC-7901/5-FU cells were treated with RES at concentrations of 0 μmol/L, 10 μmol/L, 50 μmol/L, 200 μmol/L and 400 μmol/L, respectively, for 24 h, 48 h and 72 h, and then SGC-7901/5-FU cells were treated, with RES (100 μmol/L) and 5-FU (5 μmol/L), respectively or simultaneously, for 48 h. The cell proliferation was detected by CCK-8 method, the apoptosis by flow cytometry, and the cell morphology by immunofluorescence staining method. The mRNA expression levels of MDR1, Bcl2 and Caspase 3 were detected by RT-PCR, and the protein expression levels of P-gp, Bcl2 and caspase 3 were detected by Western blot. Results RES with different concentrations could inhibit the proliferation of SGC-7901/5-FU cells effectively, depending upon time and dose. The RES group, 5-FU group and combined group could induce apoptotic and morphology changes in SGC-7901/5-FU cells, which was most remarkable in the combined group(P<0.001). The expression levels of MDR1 mRNA and P-gp protein in RES group and combined group were decreased (P<0.05), which was more significant in combined group; the Bcl2 mRNA and protein expression levels were decreased in RES group, 5-FU group and combined group (P<0.001), while Caspase 3 mRNA and protein expression levels were increased (P<0.001), which was more pronounced in the combined group. Conclusions RES can inhibit the proliferation of SGC-7901/5-FU cells by down-regulating the expression of drug-resistance related gene MDR1/P-gp protein and induce cell apoptosis to reduce drug resistance.