Anti-Glioma Effect of Pseudosynanceia melanostigma Venom on Isolated Mitochondria from Glioblastoma Cells

Background: Glioblastoma is the most common primary malignant tumor of the central nervous system thatoccurs in the spinal cord or brain. Pseudosynanceia melanostigma is a venomous stonefish in the Persian Gulf, whichour knowledge about is little. This study’s goal is to investigate the toxicity of stonefish crude venom on mitochondriaisolated from U87 cells. Methods: In the first stage, we extracted venom stonefish and then isolated mitochondria haveexposed to different concentrations of venom. Finally, mitochondrial toxicity parameters (Succinate dehydrogenase(SDH) activity, Reactive oxygen species (ROS), cytochrome c release, Mitochondrial Membrane Potential (MMP),and mitochondrial swelling) have evaluated. Results: To determine mitochondrial parameters, we used 115, 230, and460 μg/ml concentrations. The results of our study show that the venom of stonefish selectively increases upstreamparameters of apoptosis such as mitochondrial swelling, cytochrome c release, MMP collapse and ROS. Conclusion:This study suggests that Pseudosynanceia melanostigma crude venom has selectively caused toxicity by increasingactive mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma.