三种全自动粪便分析仪对粪便隐血和幽门螺杆菌抗原检测的效果比较

 目的 探讨科域KU-F20、爱威AVE-562、沃文特FA280三台不同品牌型号粪便分析仪检测粪便隐血实验(occult blood test,OB)和粪便幽门螺杆菌(helicobacter pylori,HP)抗原,与人工法比较的一致性。方法 收集2020-06至2020-09住院患者粪便标本1851例,分别采用仪器法和人工法进行粪便OB和HP抗原检测,计算每台仪器的灵敏度、特异度、符合率,并进行差异比较及一致性分析。结果 KU-F20、AVE-562、FA280检测粪便OB与人工法比较,灵敏度分别为0.96、0.91、0.99,特异度分别是0.75、0.84、0.63,符合率分别是0.91、0.89、0.92,配对卡方检验分别是P=0.424、0.099、＜0.01,Kappa值分别是0.73、0.71、0.71。KU-F20、AVE-562、FA280检测粪便HP与人工法比较,灵敏度分别为0.88、0.85、0.90,特异度分别是0.60、0.95、0.66,符合率分别是0.80、0.88、0.83,配对χ²检验分别是P=0.689、＜0.01、0.56,Kappa值分别是0.48、0.73、0.56。结论 三台仪器检测粪便OB同人工法比较,一致性显著;AVE-562检测粪便HP抗原同人工法比较,一致性显著。

Comparison of three automatic stool analyzers in the detection of fecal occults and helicobacter pylori antigen

Objective To investigate the consistency between the results of fecal occult blood test and helicobacter pylori antigen test detected by KU-F20, AVE-562 and FA280 stool analyzers and those of manual detection. Methods A total of 1851 fecal samples from inpatients were collected between June and September 2020. The fecal occult blood and helicobacter pylori antigens were detected by automatic stool analyzers and manually respectively. The sensitivity, specificity and coincidence rate of each instrument were calculated, while the difference and consistency were analyzed. Results Compared with manual detection, the sensitivity of Ku-F20, AVE-562 and FA280 fecal occult blood detection was 0.96, 0.91 and 0.99 respectively, the specificity was 0.75, 0.84 and 0.63 respectively, the coincidence rate was 0.91, 0.89 and 0.92 respectively, the paired chi-square test was P=0.424, 0.099, <0.01, and the Kappa values were 0.73, 0.71 and 0.71 respectively. The sensitivity of Ku-F20, AVE-562 and FA280 for helicobacter pylori detection was 0.88, 0.85 and 0.90 respectively, the specificity was 0.60, 0.95 and 0.66 respectively, the coincidence rate was 0.80, 0.88 and 0.83 respectively, the paired chi-square test was P=0.689, <0.01, 0.56, and the Kappa values were 0.48, 0.73 and 0.56 respectively. Conclusions The consistency of the three stool analyzers with manual detection in detecting fecal occult blood is significant, so is the consistency between AVE-562 and manual detection in detecting fecal helicobacter pylori antigens.