实时动态法分析七味红花殊胜丸抑制肝癌细胞线粒体代谢

目的:观察七味红花殊胜丸对人肝癌SMMC-7721细胞线粒体代谢酶的影响.方法:运用实时动态法分析七味红花殊胜丸对细胞活性的影响及各给药组不同时间IC50值的变化情况.采用MTT法检测七味红花殊胜丸对SMMC-7721细胞的抑制率;ELISA法检测七味红花殊胜丸对SMMC-7721细胞线粒体代谢和凋亡的影响,Weste...

Real-time dynamic analysis of Qiwei Honghua Shusheng Pill inhibiting mitochondria metabolism of liver cancer cells

OBJECTIVE To observe the effect of Qiwei Honghua Shusheng Pill on SMMC-7721 hepatoma cell mitochondria.METHODS The effects of each dosing group on cellular activity and IC50 value of each timepoint were analyzed by real-time dynamic detection. Methyl thiazolyl tetrazolium (MTT) colorimetry was employed for observing the inhibition rate of Qiwei Honghua Shusheng Pill on human liver cancer SMMC-7721 cell and enzyme-linked immunosorbent assay (ELISA) for detecting the effect of Qiwei Honghua Shusheng Pill on energy metabolism and apoptosis. The expression of apoptotic protein was detected by Western blot.RESULTS The results of real-time dynamic detection indicated that 48h was the best timepoint and 5 mg·mL^-1 the best concentration. MTT implied that Qiwei Honghua Shusheng Pill could inhibit cellular proliferation after 48 h in a concentration-dependent manner (P<0.01). ELISA showed that Qiwei Honghuashusheng Pill with large/medium doses (10/5 mg·mL^-1) could lower the expressions of ATP, ATPase and mitochondrial respiratory chain complex Ⅰ/Ⅱ/Ⅲ/Ⅳ enzymes and affect cellular energy metabolism. It could also enhance the contents of pro-apoptotic proteins Bax, caspase-3 and caspase-9, down-regulate the expression of anti-apoptotic protein Bcl-2 and induce the apoptosis of liver cancer cells.CONCLUSION Qiwei Honghua Shusheng Pill may induce the apoptosis of liver cancer cells through regulating cellular energy metabolism.