根皮素醇质体的制备、表征及对体外透皮吸收的影响

目的:制备根皮素醇质体,考察醇质体作为根皮素经皮给药载体的可行性.方法:乙醇注入法制备根皮素醇质体.采用包封率和粒径为考察指标,正交试验优化大豆磷脂用量、胆固醇用量、乙醇体积及水浴温度.测定根皮素醇质体粒径分布、多分散指标和Zeta电位,采用Franz扩散池比较根皮素及其醇质体体外透皮特征.结果:根皮素醇质体最佳处方工...

Phloretin ethosome: preparation,characterization and effects on skin penetration in vitro

OBJECTIVE To prepare phloretin ethosomes and examine its feasibility as a transdermal delivery carrier for phloretin in vitro. METHODS Phloretin ethosomes were prepared by ethanol injection.Encapsulation efficiency and particle size were employed as parameters.Orthogonal test was utilized for optimizing the dosage of phospholipids,cholesterol,ethanol as well as temperature of water bath.Particle size distribution,polydispersity index(PDI)and Zeta potential of ethosomes were determined.Franz diffusion cells were utilized for comparing skin penetration properties of phloretin and its ethosomes. RESULTS The optimal formulations were phospholipids 220 mg,cholesterol 14 mg and ethanol 5 mL with a temperature of water bath 40℃.Encapsulation efficiency,drug loading,average particle size,PDI and zeta potential of phloretin ethosomes were(83.09±1.24)%,(4.86±0.89)%,(162.19±5.88)nm,(0.067±0.011)and(-15.09±2.16)mV respectively.The cumulative permeated drug quantity per unit area of phloretin ethosomes was boosted 3.43 times and permeation process was closer to zero-order kinetics.CONCLUSION The prepared ethosomes have the advantages of high encapsulation efficiency and uniform particle size,and could enhance permeation rate and accumulative penetration of phloretin.Further researches are required.