基于液相质谱技术探讨阿霉素诱导扩张型心肌病潜在血浆标志物

目的:扩张性心肌病是应用阿霉素治疗肿瘤的严重副作用之一,致死率可达50％.通过建立阿霉素扩张型心肌病动物模型对比健康动物,寻找该疾病的生物标志物.方法:采用阿霉素注射法制备扩张型心肌病模型,造模后收集模型组与对照组大鼠的血浆与心肌组织,应用液相质谱技术检测血浆及心肌代谢物,选择共同存在于血浆及心肌组织中的代谢物,并应用...

Potential plasma biomarkers of adriamycin-induced dilated cardiomyopathy based upon UHPLC-MS/MS

OBJECTIVE Dilated cardiomyopathy is one of the severe side effects for adriamycin in the treatment of cancers.The mortality rate was as high as 50%.Biomarkers were searched by comparing animals in control and adiramycin-induced cardiomyopathy groups. METHODS Animals in adiramycin-induced cardiomyopathy group received an injection of adriamycin.Plasma and cardiac tissue were collected when animals in adiramycin-induced cardiomyopathy group were diagnosed with dilated cardiomyopathy.Liquid chromatography-mass spectrometry was utilized for examining metabolites and ROC curve was plotted for selecting biomarkers.RESULTS A total of 45 metabolites were discovered both in plasma and cardiac tissue.And 22 of them had an AUC value of 1 as well as great specificity and sensitivity. CONCLUSION With potent diagnostic values,these 22 metabolites,including deoxyuridine,may become potential biomarkers for adriamycin-induced dilated cardiomyopathy.