沉默环状RNA hsa_circ_0000591表达对肝癌细胞增殖及迁移的影响

目的探讨环状RNA hsa_circ_0000591在肝细胞癌(hepatocellular carcinoma,HCC)组织以及肝癌细胞系中的表达,及其对肝癌细胞增殖和迁移的影响。方法收集2014—2018年广西医科大学附属肿瘤医院手术切除的84例HCC组织及其相应癌旁正常组织标本,采用qRT-PCR检测hsa_circ_0000591的表达,并分析其与预后的关系。将hsa_circ_0000591沉默慢病毒及阴性对照慢病毒感染肝癌Hep3B细胞,记为siRNA组和NC组。采用CCK-8实验、平板克隆形成实验检测细胞增殖能力;划痕实验检测细胞迁移能力;Transwell实验检测细胞侵袭和迁移能力。结果qRT-PCR检测结果显示,hsa_circ_0000591在HCC组织中的表达高于癌旁组织(P<0.001),在肝癌细胞系Li-7、HepG2、Hep3B、Huh7及人体正常肝细胞系HL-7702中的表达差异有统计学意义(P<0.05),其中在Hep3B细胞中的表达量高于其余肝癌细胞系及正常肝细胞系HL-7702(均P<0.05)。与NC组比较,沉默hsa_circ_0000591后肝癌Hep3B细胞中的hsa_circ_0000591表达下调(P<0.05),细胞增殖、迁移和侵袭能力降低(P<0.05)。结论hsa_circ_0000591在肝癌中表达上调,沉默hsa_circ_0000591可抑制肝癌细胞增殖和迁移,hsa_circ_0000591可能是肝癌潜在的分子靶点。

Effect of silencing circular RNA hsa_circ_0000591 expression on proliferation and migration of liver cancer cells

 Objective To investigate the expression of circular RNA hsa_circ_0000591 in hepatocellular carcinoma (HCC) tissues and liver cancer cell lines, and its effect on the proliferation and migration of liver cancer cells. Methods A total of 84 cases of HCC tissues and corresponding normal adjacent liver tissues between 2014 and 2018 were collected from Guangxi Medical University Cancer Hospital. The expression of hsa_circ_0000591 was detected by qRT-PCR, and its relationship with the prognosis was analyzed. The silenced hsa_circ_0000591 lentivirus and the negative control lentivirus were used to infect Hep3B cells, which were recorded as the siRNA group and the NC group, respectively. The cell proliferation ability was detected by CCK-8 assay and plate cloning assay; the cell migration ability was detected by scratch test; the invasion and migration ability was detected by Transwell test. Results The results of qRT-PCR showed that the expression of hsa_circ_0000591 in HCC tissues was higher than that in adjacent tissues (P<0.001), the expression of hsa_circ_0000591 in liver cancer cell lines Li-7, HepG2, Hep3B and Huh7 and human normal liver cell lines HL-7702 was statistically significant (P<0.05), and the expression of Hep3B was higher than that of other liver cancer cell lines and normal liver cell line HL-7702 (all P<0.05). Compared with the NC group, the expression of hsa_circ_0000591 in Hep3B cells was decreased after silencing hsa_circ_0000591 (P<0.05), and the ability of proliferation, migration and invasion were significantly reduced (P<0.05). Conclusions The expression of hsa_circ_0000591 is up-regulated in liver cancer. Silencing hsa_circ_0000591 can inhibit the proliferation and migration of liver cancer cells. hsa_circ_0000591 may be a potential molecular target for liver cancer.