桃红四物汤醇提液的薄层分离与定性鉴别

目的：桃红四物汤醇提液的薄层分离与定性鉴别。方法：采用薄层色谱法，选用硅胶G 薄层板，以正丁醇- 冰乙酸- 水（4∶1.5∶5，上层）为展开剂，置紫外灯（365 nm）下检视，观察桃红四物汤醇提液中化学成分的展开情况，再选用碘蒸气对各斑点进行显色，并将薄层板置于薄层扫描仪进行扫描，记录各斑点的吸收光谱图与比移值，与对照品进行观察对比。结果：桃红四物汤醇提液共分离得到5 个斑点，其中定性鉴别出3 个斑点，分别为来自红花的羟基红花黄色素A、来自白芍的芍药苷、来自当归和川芎的阿魏酸；其它2 个斑点在组成桃红四物汤的六个单味药中均未检出。结论：薄层色谱法能实现对桃红四物汤醇提液中化学成分的分离，可在此基础上进一步进行新成分的确定，分离方法简单快速、稳定可行。

Thin-layer Separation and Qualitative Identification of the Alcohol Extract of Taohong Siwu Decoction

Objective：Thin layer separation and qualitative identification of alcohol extract of Taohong Siwu decoction. Methods：Thin layer chromatography （TLC） was used to observe the development of chemical components in the alcohol extract of Taohong Siwu decoction，and iodine vapor was used to perform color rendering on the spots，and the thin layer plate was placed in a thin-layer scanner for scanning. The absorption spectra and retardation factor （Rf）values of the spots were recorded and compared with the reference substances. The silica gel G thin laminate was used as the stationary phase，and n-butanol-acetic acid-water （4∶1.5∶5，upper layer） was used as the developing agent，and examined under UV lamp （365 nm）. Results：A total of 5 spots were isolated from the alcohol extract of Taohong Siwu decoction，among which 3 spots were qualitatively identified as hydroxyl safflower yellow A （from Carthami Flos），paeoniflorin（from Paeoniae Radix Alba），and ferulic acid （from Angelicae Sinensis Radix and ChuanXiong Rhizoma）. The other 2 spots were not detected in the six single drugs of Taohong Siwu decoction. Conclusion：TLC can be used to separate the chemical constituents from the alcohol extract of Taohong Siwu decoction，and on this basis，new constituents can be further identified. The separation method is simple，rapid，stable，and feasible.