Enhanced protection against Q fever in BALB/c mice elicited by immunization of chloroform-methanol residue of Coxiella burnetii via intratracheal inoculation |
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| Affiliation: | 2. Anhui Medical University, Mei-Shan Road, Hefei, Anhui 230032, China;1. Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran;2. Department of Biostatistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran;3. Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;4. Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran;5. Isfahan Veterinary Offices, Head of Public Health Supervision Section, Isfahan, Iran;1. National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, China;2. Changchun BCHT Biotechnology Co., Changchun 130012, China;3. Mucosis B.V., L.J. Zielstraweg 1, 9713 GX Groningen, The Netherlands;4. College of Life Science, Jilin Agricultural University, Changchun 130118, China;1. Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;2. Graduate from School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;3. Department of Microbiology, khoramabad University of Medical Sciences, Khoramabad, Iran;1. School of Pharmacy, the Key Laboratory of Prescription Effect and Clinical Evaluation of State Administration of Traditional Chinese Medicine of China, Binzhou Medical University, Yantai 264003, PR China;2. Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China;3. Department of Urology, the First Affiliated Hospital of Anhui Medical University, Institute of Urology, Anhui Medical University and Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui 230022, PR China;4. Department of Dental Implant Center, Stomatologic Hospital & College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei 230032, PR China;1. State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China;2. National Joint Local Engineering Research Center for Shale Gas Exploration and Development, Chongqing Institute of Geology and Mineral Resources, Chongqing, 400042, China |
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| Abstract: | Human Q fever is recognized as a worldwide public health problem. It often occurs by inhalation of airborne aerosols contaminated with Coxiella burnetii, a gram-negative intracellular bacterium, mainly from domestic livestock. In this study, we analyzed the possibility to establish mucosal and systemic immunity against C. burnetii infection using a pulmonary delivery of chloroform-methanol residue of C. burnetii (CMR) vaccine. Mice were immunized by the intratracheal inoculation of CMR (IT-CMR) or the subcutaneous injection of CMR (SC-CMR), and the immunized mice were challenged with C. burnetii by the intratracheal route. The levels of IFN-γ, IL-12p70, IL-5, and IL-4 in the IT-CMR group in splenic T cells stimulated ex vivo were significantly higher than in the SC-CMR group. Significantly elevated sIgA to C. burnetii was detected in the bronchoalveolar lavage fluid of mice immunized by IT-CMR but not by SC-CMR, which might have contributed to the significant reduction in C. burnetii load and pathological lesions in the lungs of the mice after the challenge of C. burnetii. These results suggest that compared with SC-CMR in mice, IT-CMR was more efficient to elicit cellular and lung mucosal immune responses against aerosol infection of C. burnetii. |
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| Keywords: | Q fever Intratracheal inoculation Mucosal immunity |
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