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Assessment of the efficacy of an attenuated live marker classical swine fever vaccine (Flc-LOM-BErns) in pregnant sows |
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| Institution: | 1. Virus Disease Division, Animal and Plant Quarantine Agency, Gimchen, Gyeongbuk-do, 39660, South Korea;2. College of Veterinary Medicine, Seoul University, Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea;1. Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba;2. IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain;3. Institute of Virology and Immunology IVI, Mittelhäusern, Switzerland;4. EU and OIE Reference Laboratory for Classical Swine Fever, Institute of Virology, Department of Infectious Diseases, University of Veterinary Medicine, Hannover, Germany;5. Departamentd''Agricultura, Ramaderia, Pesca, Alimentació i Medi Natural, (DAAM), Generalitat de Catalunya, Spain;6. Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Research Group Virus Genomics, Hamburg, Germany;7. Bioinformatics Service Facility, University Medical Center Hamburg–Eppendorf, Hamburg, Germany;8. Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg–Eppendorf, Hamburg, Germany;1. Animal Virology Laboratory, School of Life Sciences, BK21 plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 41566, Republic of Korea;2. ChoongAng Vaccine Laboratories, Daejeon, 34055, Republic of Korea;3. Department of Neural Development and Disease, Korea Brain Research Institute, Daegu, 41068, Republic of Korea;4. Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea;1. National Food Chain Safety Office, Directorate of Veterinary Medicinal Products, Budapest, Hungary;2. Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Insel Riems, Germany;3. National Veterinary Institute, Uppsala, Sweden;4. National Food Chain Safety Office, Veterinary Diagnostic Directorate, Budapest, Hungary;5. CODA-CERVA, Veterinary and Agrochemical Research Centre, Ukkel, Belgium;1. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China;2. Wuhan Chopper Biology Co., LTD, Wuhan 430070, China;1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, China;2. Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China;3. Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China;4. Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China |
| Abstract: | Here, we constructed an attenuated live marker classical swine fever (CSF) vaccine (Flc-LOM-BErns) to eradicate CSF. This was done by taking infectious clone Flc-LOM, which is based on an attenuated live CSF vaccine virus (LOM strain), and removing the full-length classical swine fever virus (CSFV) Erns sequences and the 3′ end (52 base pairs) of the CSFV capsid. These regions were substituted with the full-length bovine viral diarrhoea virus (BVDV) Erns gene sequence and the 3′ end (52 base pairs) of the BVDV capsid gene. Sows were vaccinated with the Flc-LOM-BErns vaccine 3 weeks before insemination and then challenged with virulent CSFV at the early, mid- or late stages of pregnancy. We then examined transplacental transmission to the foetuses. Piglets born to sows vaccinated with Flc-LOM-BErns did not show vertical infection, regardless of challenge time. In addition, CSFV challenge did not affect the delivery date, weight or length of the foetus. Pregnant sows inoculated with the Flc-LOM-BErns vaccine were anti-CSF Erns antibody-negative and anti-BVDV Erns antibody-positive. Challenge of pregnant sows with virulent CSFV resulted in anti-CSF Erns antibody positivity. These results strongly indicate that differential diagnosis can be conducted between the Flc-LOM-BErns vaccinated animal and virulent CSFV affected animal by detecting antibody against BVDV Erns or CSF Erns gene. Therefore, the Flc-LOM-BErns vaccine may fulfil the function of differential diagnosis which required for DIVA vaccine. |
| Keywords: | Classical swine fever virus Transplacental transmission Pregnant sow BVDV"} {"#name":"keyword" "$":{"id":"k0035"} "$$":[{"#name":"text" "_":"bovine viral diarrhoea virus CPE"} {"#name":"keyword" "$":{"id":"k0045"} "$$":[{"#name":"text" "_":"cytopathic effects CSF"} {"#name":"keyword" "$":{"id":"k0055"} "$$":[{"#name":"text" "_":"classical swine fever DIVA"} {"#name":"keyword" "$":{"id":"k0065"} "$$":[{"#name":"text" "_":"differentiating infected from vaccinated animals END"} {"#name":"keyword" "$":{"id":"k0075"} "$$":[{"#name":"text" "_":"exaltation of Newcastle disease DPI"} {"#name":"keyword" "$":{"id":"k0085"} "$$":[{"#name":"text" "_":"days post-infection ICS"} {"#name":"keyword" "$":{"id":"k0095"} "$$":[{"#name":"text" "_":"immunocytochemical staining NDV"} {"#name":"keyword" "$":{"id":"k0105"} "$$":[{"#name":"text" "_":"Newcastle disease virus nt"} {"#name":"keyword" "$":{"id":"k0115"} "$$":[{"#name":"text" "_":"nucleotide OIE"} {"#name":"keyword" "$":{"id":"k0125"} "$$":[{"#name":"text" "_":"World Organisation for Animal Health qRT-PCR"} {"#name":"keyword" "$":{"id":"k0135"} "$$":[{"#name":"text" "_":"real time polymerase chain reaction SNT"} {"#name":"keyword" "$":{"id":"k0145"} "$$":[{"#name":"text" "_":"serological neutralisation antibody test ST"} {"#name":"keyword" "$":{"id":"k0155"} "$$":[{"#name":"text" "_":"swine testicular cell 50% tissue culture infective dose WPV"} {"#name":"keyword" "$":{"id":"k0175"} "$$":[{"#name":"text" "_":"weeks post-vaccination 5′ UTR"} {"#name":"keyword" "$":{"id":"k0185"} "$$":[{"#name":"text" "_":"5′ untranslated region |
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