有毒草药粉末六角莲的鉴定与分析

目的研究未知有毒中药粉末六角莲的鉴定与分析方法。方法显微鉴别、薄层色谱法、高效液相色谱法鉴别。结果显微特征与六角莲粉末特征相同；薄层色谱显示在与对照药材及对照品色谱相应的位置上显相同颜色的斑点；液相色谱显示样品主峰的保留时间与对照药材及对照品一致。结论此三种方法能够有效鉴别六角莲；高效液相色谱法能将其有效成分鬼臼毒素和山奈素完好分离，为今后进行较详细的指纹图谱研究、含量测定研究和体液分析打下基础。

Identification and Analysis of Unknown Toxic Herbal Medicinal Powders:Dysosma pleiantha

OBJECTIVE To develop a method for identification and analysis of the unknown toxic herbal medicinal powders: Dysosma pleiantha(Hance) Woodson.METHODS By means of microscopic identification and physio-chemical analysis such as TLC,and HPLC.RESULTS The unknown powders had the same microscopic character with Dysosma pleiantha(Hance) Woodson,and the same chromatograms with reference substance of Dysosma pleiantha(Hance) Woodson reference substance and standard reference substances of kaempferol and podophyllotoxin by TLC and HPLC.CONCLUSION The established method can effectively identify the unknown herbal powders.The established HPLC method completely separates kaempferol and podophyllotoxin in Dysosma pleiantha(Hance) Woodson and this method can be used to fingerprint chromatogram research,quantitative analysis of the herbs and their residues in body.