丹参酮对维甲酸致小鼠骨质疏松的防治作用

目的:研究丹参总酮对维甲酸致小鼠骨质疏松的防治作用,并探讨其主要作用机制。方法:维甲酸(90 mg.kg-1)致小鼠骨质疏松模型,丹参酮(40,80,160 mg.kg-1)灌胃给药,实验过程监测体重,2周后以比色法测定血清钙、磷含量和碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)活性,竞争放射免疫法测定血清中骨钙素(BGP)和雌二醇(E2)含量,取股骨进行骨组织形态学观察。结果:维甲酸可引起高转换型骨质疏松,丹参酮可升高E2值,降低P,ALP,TRAP,BGP水平。结论:丹参酮对维甲酸致小鼠骨质疏松有一定的防治作用,其机制可能与其减缓雌激素水平降低,抑制骨高转换有关。

Effect of tanshitone on prevention and treatment of retinoic acid induced osteoporosis in mice

Objective : To observe the prevention and therapeutic effects of tanshitone (TAN) on retinoic acid induced osteoporosis in mice. Method : The mice osteoporosis was induced by given retinoic acid intragastrically for two weeks. The histomorphological features of bone were observed and biochemical indexes in serum (Ca, P, ALP, TRAP, E₂, BGP) were determined after the mice were given TAN at the dose of 40, 80, 160 mg·kg^-1 respectly. Result : Tanshinone can induce high conversion of osteoporosis. The levels of P, ALP, TRAP and BGP in the TAN groups were lower than the model group, while the E₂ level was higher than the model group. Conclusion : Tanshitone can prevent the loss bone in the experimental mice. The machanism may be that it improves the level of estrogenic hormone and inhibits the high bone turnover.