新型维甲酸AHPN对非小细胞肺癌H460 细胞凋亡诱导作用的实验研究

目的通过体外实验评价新型维甲酸AHPN对人肺癌细胞的抑制作用。方法应用MTT分析法、流式细胞术、形态学观察AHPN对人肺癌H460细胞的作用。结果AHPN对H460细胞的生长具有抑制作用，生长抑制率有剂量和时间依赖关系。流式细胞仪分析结果显示H460细胞有G0～G1期阻滞和细胞凋亡发生。人肺癌H460细胞在AHPN的作用下，发生了细胞凋亡的形态学改变。结论AHPN能够诱导H460细胞发生凋亡，且凋亡的发生与细胞周期阻滞相关；随浓度和时间的延长，G0-G1期细胞的比例增高。

The Research of Inhibition of Proliferation and Induction of Apoptosis of AHPN in Human Non-Small Cell Lung Carcinoma Cell Line H460 in Vitro

Objective To assess the inhibition of proliferation and the induction of apoptosis of AHPN on human non-small cell lung carcinoma cell in vitro. Methods Cell morphology, MTT assay and flow cytometry were employed to examine AHPN-induced apoptosis and growth inhibition in human lung carcinoma H460 cell. Results Cells treated with AHPN at different concentrations change in morphology. The growth inhibition effect of AHPN on H460 cells indicate time-dependence and dosage-dependence. The result of flow cytometry showed cells were blocked in G0-G1 phase and were induced to apoptosis. Conclusions AHPN can induce apoptosis of H460 cells and G0-G1 phase blockage.