巴曲酶对缺血再灌注后凋亡相关基因时效研究

目的:探讨巴曲酶对沙土鼠前脑缺血再灌注损伤后海马CA1区神经元凋亡相关基因Bcl-2、Bax表达的影响及可能的作用机制。方法:分组采用双侧颈总动脉夹闭5min后再通,造成沙土鼠前脑缺血再灌注的损伤模型,在不同时间点腹腔注射巴曲酶对其进行治疗后,运用免疫组织化学方法,检测沙土鼠海马CA1区神经元中的Bcl-2、Bax免疫反应阳性细胞的数量。结果:与对照组相比,治疗组海马CA1区Bet-2表达明显增加,Bax表达降低,尤以缺血再灌注前6h、即刻、缺血再灌注后1h、3h及6h明显(P<0.01)。结论:巴曲酶可减少脑缺血再灌注后神经细胞的凋亡,发挥脑保护作用;其作用存在明显时效关系,机理可能与增强Bcl-2的表达及抑制Bax的表达有关。

Relationship between Time and Effect of Batroxobin on Apoptosis-related Gene after Cerebral Ischemia/Reperfusion in Gerbils

Objective: To discuss the effect and possible mechanism of Batroxobin on expression of apoptosis-related gene Bcl-2 and Bax in hippocampal CA1 region after cerebral ischemia in gerbils. Methods: Gerbils were treated with Batroxobin injection through abdominal after cerebral ischemia/reperfusion(I/R) and then immunohistochemistry was used to detect the expression of Bcl-2 and Bax in neuron of hippocampal CA1 region. Results: Compared with the control group, the expression of Bcl-2 in hippocampal CAI region increased obviously, while the expression of Bax in treated group decreased, The best effects were seen from 6 hours before I/R to 6 hours after I/R. Conclusion: Batroxobin injection may reduce the apoptosis of neuron after I/R through enhancing the expression of Bcl-2 and inhibiting the expression of Bax. The effect of Batroxobin injection on cerebral I/R was time dependent.