| BGM细胞,Hela细胞,HEL细胞与A549细胞对HSV-1敏感性的比较 |
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| 引用本文： | 肖扬名,胡昌智,罗敏华,代橄,施凯. BGM细胞,Hela细胞,HEL细胞与A549细胞对HSV-1敏感性的比较[J]. 中南大学学报(医学版), 1997, 0(5) |
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| 作者姓名： | 肖扬名 胡昌智 罗敏华 代橄 施凯 |
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| 作者单位： | Department of Microbiology，Hunan Medical University，Changsha 410078 |
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| 摘 要： | 用单纯疱疹病毒－１型（ＨＳＶ－１）感染ＢＧＭ细胞、Ｈｅｌａ细胞、ＨＥＬ细胞及Ａ５４９细胞，利用空斑试验，比较四种细胞对ＨＳＶ－１的敏感性。结果示，ＨＳＶ－１在ＢＧＭ细胞培养中空斑形成单位（ＰＦＵ）数最多，出现细胞病变的时间早。提示ＢＧＭ细胞可为ＨＳＶ－１的分离培养提供一种新的、敏感细胞。
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| 关 键 词： | 人疱疹病毒；BGM细胞；空斑测定；细胞，培养的 |
THE COMPARISON OF SENSITIVITY OF BGM CELLS, HELA CELLS, HEL CELLS AND A 549 CELLS TO HERPES SIMPLEX VIRUS 1 |
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| Xiao Yangming Hu Changzhi Luo Minhua Dai Gan Shi Kai. THE COMPARISON OF SENSITIVITY OF BGM CELLS, HELA CELLS, HEL CELLS AND A 549 CELLS TO HERPES SIMPLEX VIRUS 1[J]. Journal of Central South University. Medical sciences, 1997, 0(5) |
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| Authors: | Xiao Yangming Hu Changzhi Luo Minhua Dai Gan Shi Kai |
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| Abstract: | This study was undertaken to compare sensitivity of buffulo green monkey(BGM) cells, Hela cells, human embryo lung(HEL) cells and A549 cells to herpes simplex virus 1. The plague forming unit(PFU) technique was used to measure the four cell lines simultaneously. The results showed that BGM cells were superior to the Hela cells, HEL cells, A549 cells in terms of both sensitivity and rapidity of appearance of cytopathic effect(CPE). It indicates that BGM cells constitute a new, sensitive and valuable cell line used for the detection of HSV 1 in cell culture. |
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| Keywords: | human herpes virus BGM cells * plaque assay cells cultured |
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