大肠癌组织中胸腺素β4 基因表达及其临床意义

 目的 探讨大肠癌组织中胸腺素胆基因的表达及其临床意义。方法 采用逆转录一聚合酶链反应（RT-PCR）方法，检测41例大肠癌患者肿瘤组织及正常黏膜组织中胸腺素胆基因mRNA的表达。结果 半定量RT-PCR显示所有癌组织中均有胸腺素胆mRNA表达，但在部分正常黏膜组织中表达缺失。癌组织平均表达量为0．65±0．21，正常黏膜组织为0．42±0．19（P〈0．01）。其表达与淋巴结转移和临床分期有关，在有淋巴结转移组中胸腺素胆mRNA表达（0．82±0．23）明显高于无淋巴结转移组（0．53±0．19）（P〈0．05）；C＋D期组中胸腺素胆mRNA表达（0．82±0．22）明显高于A＋B期组（0．51±0．18）（P〈0．05），与肿瘤大小、分化程度、患者性别、年龄、肿瘤部位无关。结论 大肠癌组织中胸腺素陋的表达与肿瘤的转移播散相关，胸腺素陋可作为判断大肠癌转移播散和临床分期的参考指标。

Expression of Thymosin β4 Gene in Human Colorectal Cancer Tissues and its Clinical Significance

Objective 　To study the expression of thymosin β4 in colorectal cancer and it s clinical significance. Methods 　Forty-one cases of human colorectal cancer specimens was collected f rom the patients ho received operation for colorectal cancer from 12/ 2003 to 11/ 2004. All of the specimens were determined for mRNA expression of thymosin β4 by using RT-PCR technique. Results 　Thymosin β4 mRNA was high-expressed in colorectal cancer specimens although they were also expressed in normal colorectal specimen. The result s of semi-quantitative PT-PCR analysis showed the expression of thymosin β4 mRNA was 0. 65 ±0. 21 and 0. 42 ±0. 19 in cancer and normal tissues , respectively , P < 0. 01. The relative values of mRNA in lesions with metastatic lymph nodes (0. 82 ±0. 23) was higher than those of non-metastatic lymph nodes (0. 53 ±0. 19) ( P < 0. 05) ; expression of thymosin β4 in Duke C or Duke D stage(0. 82 ±0. 22) was significantly higher than that in Duke A or Duke B stage (0. 51 ±0. 18) . There was a close relation between thymosinβ4 mRNA expression and several clinicopathological factors including ymph node metastasis , and TNM stage. Conclusion 　Thymosin β4 gene expression in colorectal cancer may serve as a parameter for judging tumor biological characteristic and clinical significance.