慢性乙型肝炎病毒基因型与血清病毒载量的关系

目的探讨徐州地区慢性乙型肝炎（CHB）患者血清中乙型肝炎病毒（HBV）基因型与病毒载量的关系。方法采用型特异性引物多重PCR方法对徐州地区160例CHB患者的HBV进行基因分型，用荧光定量PCR方法检测HBVDNA水平。结果B型14例（8．7％），C型88例（55．0％），BC混合型51例（31．9％），未分型7例（4．4％），未发现A、D、E、F型。不同基因型之间HBVDNA载量无明显差别。结论徐州地区CHB患者中HBV基因型主要是C型和BC混合型，感染的HBV基因型与血清病毒载量之间无明显相关。

The relationship between virus genotype and HBV DNA level in patients with chronic hepatitis B

Objective To explore the relationship between viral genotype and serum HBV DNA level in patients with chronic hepatitis B(CHB) in Xuzhou region.Methods The viral genotype was identified by special primer multiple PCR and the HBV DNA level was determined by quantitative PCR on fluorescence assay.Results In the 160 patients with CHB,genotype B was detected in 14 cases(8.7%),genotype C in 88 cases(55.0%),and mixed genotype of B and C in 51 cases(31.9%).7 cases failed the genotyping(4.4%).Genotypes A,D,E and F were not found in the study.No significant deviation in HBV DNA level was noticed among different genotype groups.Conclusion The predominant genotypes of HBV in patients with CHB in Xuzhou region are C and mixed B and C;no significant relation was found between the viral genotype and the serum HBV DNA level in patients with CHB.