基因芯片技术及其在医学领域中的应用新进展

基因芯片技术是伴随人类基因组计划的实施而发展起来的一门新兴技术,该技术将成千上万个DNA或寡核苷酸片段固定在玻璃、尼龙膜或硅片等载体上,与标记的样品探针杂交,分析样品中基因表达、基因序列、基因突变和多态性变化等情况,这是一个高效率和大规模的基因组分析和基因表达的研究技术。可用于疾病特别是肿瘤分化、分型、诊断、发病机制、疗效观察和发现新的肿瘤相关基因等研究领域。

Gene chip technology and its advances in medical science

Gene(DNA)chip or DNA microarray is a new technique developed during human genomic rese-arch programs.When DNA chip containing thousands of DNA or oligonu cleotide are hybridized to lab-eled samples,gene expression,DNA sequencing as well as DNA mutation and polymorphism can be analyzed with high efficiency in la rge scale.We are able to perform this technique on dozens of specimens at once in tumor differentiation,tumor typing,tumor diagnosis,tumorigenesis,and new tum-or-associated genes discovery.