二十五味珊瑚丸中马兜铃酸A含量研究

目的:建立二十五味珊瑚丸中马兜铃酸A含量的HPLC测定方法,用于市场上二十五味珊瑚丸中马兜铃酸A的定性和定量检测。方法:采用kromasil C18(4.6×200mm),甲醇-0.05%冰醋酸(62:38)作为流动相,柱温30℃,流速1mL/min,250nm波长下检测。结果:马兜铃酸A在0.052mg/mL-0.0936mg/mL范围内线性关系良好,r=0.9999。平均回收率为99.67%,RSD为1.33%。结论:以高效液相色谱法检测二十五味珊瑚丸中马兜铃酸A的方法简便、快速、准确、灵敏度高,重复性良好。

Determination of Aristolochic Acid A in Ershiwuweishanhu Pill by HPLC

Objective： To develop a high performace liquid chromatography （HPLC） method for the determination of aristolochic acid A in Ershiwuweishanhu Pill and to use the method in the qualitative and quantitative determination of aristolochic acid A in Ershiwuweishanhu Pill purchased from market. Methods： The column used was kromasil C18 （4.6 x 200 ram）, and methanol -0.05% glacial acetic acid （62：38） was used as the mobile phase. The column temperature was set at 30~C, the flow rate was 1 mL/min and the detection wavelength was set at 250 nm. Resallts： The calibration curve of aristolochic acid A was linear in the range of 0.052-0.0936 mg/mL （r= 0.9999）. The average recovery was 99.67%. RSD wass 1.33%. Conclusion： The established method is convenient, fast, accurate, sensitive and repeatable.