化疗+G-CSF方法动员外周血干细胞时骨髓及外周血相关细胞亚群的动态变化

目的探讨应用化疗 G-CSF方法动员外周血干细胞时,恶性血液病患者的骨髓及外周血CD34 细胞和T细胞亚群的变化特点.方法 16例拟行自体外周血干细胞移植患者,在应用化疗 G-CSF方法动员外周血干细胞期间,应用流式细胞仪测定骨髓及外周血CD34 和T细胞亚群动态变化情况.结果 1.恶性血液病患者在应用G-CSF前,骨髓中CD34 细胞的初始值为(0.52±0.31)%.应用G-CSF 48 h后,CD34 细胞测定值为(1.22±0.42)%,较前有明显增加(P＜0.001).CD34 细胞的高峰值出现在应用G-CSF 96 h后,为(2.23±0.34)%,较未应用G-CSF时差异显著(P＜0.001).2.恶性血液病患者在应用G-CSF前,外周血CD34 细胞测定值为(0.39±0.27)%.在应用G-CSF 72 h后外周血中CD34 细胞的测定值为(1.29±0.64)%,较前有明显增加(P＜0.001).应用G-CSF96 h后,CD34 细胞的测定值达高峰,为(1.41±0.73)%,较未应用G-CSF前差异显著(P＜0.001).3.无论是否应用G-CSF,恶性血液病患者的T淋巴细胞亚群比例均处于倒置状态,应用G-CSF后随CD34 细胞的逐渐增加,T淋巴细胞亚群变化不明显(P＞0.05).结论恶性血液病患者,在应用化疗 G-CSF动员外周血干细胞时,其骨髓及外周血CD34 细胞和T细胞亚群的动态变化各有其特点.

Effect of chemotherapy plus G- CSF on bone marrow or peripheral blood CD34 + cell and T lymphycyte subsets

Objective: To approach what changes happen about CD34 + cells and T lymphocyte subsets after using chemotherapy plus G - CSF with patients. Methods: Flow cytometry was used to detect the changes of the percentages of CD34 + cells and T lymphocyte cell subsets in 16 cases of patients when they were treated with chemotherapy and injected with G - CSF for mobilizing peripheral blood stem cells. Results: 1. Without using G - CSF, the percentages of CD34 + cells in the bone marrow of the patients ranged in (0.52 ± 0.31) %. After Using G - CSF for 48hrs, the percentages ranged in (1.22±0.42)%,obviously increased compared with those of no using G - CSF (P＜0.001).After 96hrs the highest percentages were reached and ranged in (2.23 ± 0.34) % , significant increased compared with those of no using G - CSF (P＜0.001). 2. Without using G - CSF, the percentages of CD34 + cells in peripheral blood of the patients ranged in (0.39 ± 0.27 ) %. After using G - CSF for 72 hours, they ranged in ( 1.29±0.64)%, obviously increased compared with those of no using G- CSF (P＜0.001). After 96 hrs the highest percentages were reached and ranged in (1.41±0.73) %, significant increased compared with those of no using G -CSF (P＜0.001). 3. CD4/CDs of the patients were abnormal (＜1) whether in bone marrow cells or peripheral blood cells. There were no obvious changes of T lymphocyte subsets in the patients except that CD34 + cells increased gradually(P＞0.05) when using G -CSF for some days . Conclusions:The dynamic change of the percentages of CD34 +cells and T lymphocyte subsets in bone marrow or peripheral blood of patients with CR complete remission has itself characteristics after using chemotherapy plus G - CSF for days.