| DIFFERENTIAL EXPRESSION OF GENES IN OMENTAL FAT OF NORMAL WEIGHT AND OBESE SUBJECTS AND OBESE DIABETIC PATIENTS USING eDNA MICROARRAY |
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| 引用本文： | 骆天红 赵萸 李果 张宏利 李文毅 刘优萍 罗敏 王侃侃 张济 郑培烝 赵春军. DIFFERENTIAL EXPRESSION OF GENES IN OMENTAL FAT OF NORMAL WEIGHT AND OBESE SUBJECTS AND OBESE DIABETIC PATIENTS USING eDNA MICROARRAY[J]. 上海第二医科大学学报, 2007, 19(1): 1-7,36 |
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| 作者姓名： | 骆天红 赵萸 李果 张宏利 李文毅 刘优萍 罗敏 王侃侃 张济 郑培烝 赵春军 |
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| 作者单位： | [1]Shanghai Institute of Endocrinology, Shanghai Clinical Research Center of Endocrine and Metabolic Diseases, Ruifin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China [2]Shanghai Institute of Hematology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China |
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| 基金项目： | Supported by grants from State Key Project of Tackling Scientific and Technological Problems, China （2002BA711A05）, the National Natural Sciences Foundation of China （30571019）, the Author of National Excellent Doctoral Dissertation of China （200360） and Shanghai Rising-Star Project （03QC14040）. |
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| 摘 要： | Objective To identify genes differentially expressed in omental fat of normal weight subjects, obese subjects and obese diabetic patients. Methods Using a high-density cDNA microarray, gene expression profile of omental fat from normal weigh subjects, obese subjects and obese diabetic patients were compared Results Totally, 119 and 257 genes were up-regulated in obese subjects and obese diabetic patients respectively, while 46 and 58 genes were down-regulated. A total of 77 genes, including PDK4 , which switched from carbohydrate to fatty acids as the primary source of fuel, were up-regulated in both obese and obese diabetic patients, while 8 genes, including key enzymes in lipid synthesis, such as HMG-CoA synthase, fatty acid synthase and stearoyl-CoA desaturase, were down-regulated in both groups. Tyrosine-3-monooxygenase/tryptophan 5-monooxygenase activation protein θ（ YWHAZ） , a negative regulator for insulin signal transduction, was up-regulated only in obese diabetic patient, but not in normal-glycemic obese subjects. Conclusion The study demonstrated that decrease of lipogenesis along with increase of fatty acids oxidation of adipose tissue could be a common cause of insulin resistance in obesity and type 2 diabetes, while block of insulin signal transduction may trigger the transition from obesity to diabetes. Further exploration of these genes will be useful in the understanding of the pathogenesis of obesity and diabetes.
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| 关 键 词： | 基因表达 网膜 肥胖 糖尿病 |
DIFFERENTIAL EXPRESSION OF GENES IN OMENTAL FAT OF NORMAL WEIGHT AND OBESE SUBJECTS AND OBESE DIABETIC PATIENTS USING eDNA MICROARRAY |
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| LUO Tian-hong, ZHAO Yu, LI Guo, ZHANG Hong-li, LI Wen-yi, LIU You-ping, LUO Min,WANG Kan-kan, ZHANG Ji, ZHENG Pei-zheng, ZHAO Chun-jun. DIFFERENTIAL EXPRESSION OF GENES IN OMENTAL FAT OF NORMAL WEIGHT AND OBESE SUBJECTS AND OBESE DIABETIC PATIENTS USING eDNA MICROARRAY[J]. Journal of Shanghai Second Medical University(Foreign Language Edition), 2007, 19(1): 1-7,36 |
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| Authors: | LUO Tian-hong ZHAO Yu LI Guo ZHANG Hong-li LI Wen-yi LIU You-ping LUO Min WANG Kan-kan ZHANG Ji ZHENG Pei-zheng ZHAO Chun-jun |
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| Abstract: | |
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| Keywords: | obesity diabetes microarray adipose tissue |
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