Absence of measles virus receptor (CD46) in lesions of subacute sclerosing panencephalitis brains |
| |
Authors: | Akihiko Ogata Stefanie Czub Seiko Ogata S Louise Cosby Stephen McQuaid Herbert Budka Volker ter Meulen Jürgen Schneider-Schaulies |
| |
Institution: | Institut für Virologie und Immunbiologie, Universit?t Würzburg, Versbacher Strasse 7, D-97078 Würzburg, Germany Tel.: 49-931-201 5954; Fax: 49-931-201 3934, DE Insitut für Pathologie, Universit?t Würzburg, Versbacher Strasse 3, D-97078 Würzburg, Germany, DE School of Biology and Biochemistry and Clinical Medicine, The Queen’s University of Belfast, Belfast BT9 7BL, UK, GB Klinisches Institut für Neurologie der Universit?t Wien, Klinische Abteilung für Neuropathologie und Neurochemie, W?hringer Gürtel 18–20, A-1097 Wien, Austria, AT
|
| |
Abstract: | In this study we investigated pathological changes of the expression of the measles virus (MV) receptor, CD46, in subacute
sclerosing panencephalitis (SSPE) brains. We analyzed CD46 expression in lesions of brain specimens from five SSPE patients
in comparison to uninfected regions of the same brains and to normal human brains. The correlation between CD46 and MV infection,
in individual cells in SSPE brains, was analyzed by double-staining procedures using monoclonal antibodies (mAbs) and in situ
hybridization to detect MV-specific mRNAs. We found that CD46 was expressed at relatively low levels by neurons and astrocytes
in normal brains in comparison to neuroblastoma and astrocytoma cell lines. Within heavily infected (MV-positive) brain lesions
of all five SSPE cases, CD46 was either not detected or was expressed to a lesser degree by neural cells, irrespective of
whether MV antigens were detectable or not. In contrast, normal levels of CD46 were found in SSPE brain tissue distant from
the lesion. Using in situ hybridization, mRNAs of both MV nucleocapsid and MV hemagglutinin (MV-H) were detected in all SSPE
lesions, while no or only small amounts of MV-H protein were detected. MV-infected neurons were never found to express CD46.
Although a strict correlation between levels of the MV-H protein and the absence CD46 could not be seen, these findings suggest
that the CD46 expression is reduced by the MV infection in lesions of SSPE brains.
Received: 3 March 1997 / Revised, accepted: 30 April 1997 |
| |
Keywords: | CD46 Measles virus receptor Subacute sclerosing panencephalitis Measles virus |
本文献已被 SpringerLink 等数据库收录! |
|