| 抗人IL—2单克隆抗体的制备及其特征 |
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| 引用本文： | 张叔人 常立水. 抗人IL—2单克隆抗体的制备及其特征[J]. 中国免疫学杂志, 1989, 5(4): 213-215 |
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| 作者姓名： | 张叔人 常立水 |
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| 作者单位： | 中国医学科学院肿瘤研究所免疫室，中国医学科学院肿瘤研究所免疫室，中国医学科学院肿瘤研究所免疫室，中国医学科学院肿瘤研究所免疫室 北京 100021，北京 100021，北京 100021，北京 100021 |
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| 摘 要： | 用基因重组人IL—2(rIL—2)免疫的BALB/C小鼠脾细胞与NS—1细胞株杂交,经3～5次克隆获得9株分泌抗IL—2单克隆抗体杂交瘤,所分泌抗体均为IgG_1。其中3B5杂交瘤诱生的腹水中抗体效价达10万倍稀释度。它既能中和用于免疫的rIL—2活性,也可中和其它来源的基因重组人rIL—2,但与天然rIL—2反应弱。rIL—2可与4C3抗体包被的Sepharose 4B组合,並能用1.5%醋酸洗脱。因此它可做为基因工程制备rIL—2的辅助试剂及用于与rIL—2相关的研究。
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| 关 键 词： | 单克隆抗体 IL－2 免疫亲和层析 |
Preparation and characterization of monoclonal antibody against human IL-2. |
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| Zhang Shuren,et al.. Preparation and characterization of monoclonal antibody against human IL-2.[J]. Chinese Journal of Immunology, 1989, 5(4): 213-215 |
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| Authors: | Zhang Shuren et al. |
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| Affiliation: | Zhang Shuren,et al. Cancer Institute,Chinese Academy of Medical Sciences,Beijing. |
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| Abstract: | Spleen cells of BALB/c mice immunized with recombinant human IL-2 (rIL-2) were hybridized with NS-1 myeloma cell 1ine. 9 stable clones of hybri domas secreting monoclonal antibody (McAb) against human IL-2 were obtained. The isotype of the McAb was defined as (?). The antibody titer in 3B5 clone-induced ascite was found to be higher than 10~(-5). This McAb was able to neutralize the activity of the immunizing rIL-2 (Cetus) as well as human rIL-2 (expressed in E. coli and yest) from other sources. It was found that this McAb reacted weakly with human natural IL-2 and the recombinant IL-2 avidly bond to an affinity column of Sepnarose 4B conjugated with the McAb and could be eluted with 1.5% acetic acid. Thus these McAbs are useful for the preparation of purified rIL-2. |
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| Keywords: | Monoclonal Antibody Recombinant Human Interleukin 2 Immunoaffinity |
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