RP-HPLC法测定心可舒胶囊中原儿茶醛的含量

目的：建立心可舒胶囊中原儿茶醛的HPLC测定含量方法。方法：色谱柱：phenomenesC18柱（200mm×4．6mm，51Am），流动相：甲醇-水-冰醋酸水溶液（10：90：0．5），流速：1．0ml·min^-1，检测波长：280hm。结果：原儿茶醛在0．80～4．0μg范围内有良好的线性关系（r=0．9997），平均加样回收率为99．87％（RSD：1．0％）。结论：本法简便、灵敏度高，分离效果好，适用于该制剂的质量控制。

Detemination the Content of Protocatechulc Aldehyde in Xinkeshu Capsule by RP-HPLC

Objective ： To establish a method for determination the content of protocatechuic aldehyde in Xinkeshu Capsule by RP- HPLC. Method： The phenomenes C is column （ 200mm × 4.6mm, 5 μm） was used , the mobile phase was methanol-water- glacial acetic acid（ 10： 90：0.5 ） , the flow rate was 1.0 ml. min-1 , and the detection wavelength was 280nm. Result： The linear range of protocatechuie aldehyde was 0.80 - 4.00μg （ r = 0. 999 7 ）, the average recovery was 99.87% （ RSD = 1.0% ）. Conclusion ： The method is simple, sensitive and accurate,it can be used for the quality control of Xinkeshu Capusule.