血管紧张素原基因M235T分子变异与心肌梗塞的关系

目的为探讨血管紧张素原（ＡＧＴ）基因Ｍ２３５Ｔ分子变异与心肌梗塞（ＭＩ）的关系。方法采用聚合酶链反应、限制性片段长度多态性分析，对５７例ＭＩ患者和７６例无冠心病证据的对照组进行ＡＧＴ基因Ｍ２３５Ｔ等位基因检测。结果ＭＩ患者ＡＧＴ基因２３５ＴＴ型（０．７０）和Ｔ２３５等位基因（０．８２）的频率显著高于对照组（分别为０．４２和０．６３，Ｐ＝０．０１３和Ｐ＜０．０２５）。经校正冠心病的主要危险因素后，ＡＧＴ基因２３５ＴＴ型仍可显著增加心肌梗塞发生的危险性（比数比３．６５，Ｐ＝０．０１６）。结论ＡＧＴ基因２３５ＴＴ型可能是中国人群ＭＩ发病的重要危险因素之一。

A MOLECULAR VARIANT OF ANGIOTENSINOGEN GENE IS ASSOCIATED WITH MYOCARDIAL INFARCTION IN CHINESE

Objective A molecular variant of the angiotensinogen (AGT) gene with threonine instead of methionine at position 235(i.e.,with M235T polymorphism) has been shown to be associated with essential hypertension, preeclampsia, coronary atherosclerosis, coronary heart disease(CHD), myocardial infarction (MI) in Caucasians and in Japanese. The purpose of the present study was to assess whether the M235T polymorphism was associated with MI in a Chinese population. Methods M235T polymorphism in exon 2 of AGT gene was determined by polymerase chain reaction(PCR) and restriction endonuclease analysis in a study of 57 patients with MI and 76 non CHD individuals as control. Results The frequencies of T235 allele(0.82) and 235TT genotype(0.70) in the MI group were higher than those in the control subjects(0.63 and 0.42 respectively, P =0 013 and P <0 025). AGT gene 235TT genotype was at significantly increased risk of MI(odds ratio 3.65, P =0.016) in analysis adjusted for several main CHD risk factors. Conclusion There is a significant association between AGT gene 235TT genotype and MI, this genotype might be an independent risk for MI in Chinese population.