槲皮素对高糖损伤血管内皮细胞的保护作用

目的:研究槲皮素对高糖损伤血管内皮细胞(VECs)的保护作用。方法:实验分对照组,损伤组和槲皮素保护组。用含10 mg/ml葡萄糖、20％胎牛血清的DMEM培养基离体培养 VECs。MTT比色法测细胞存活数量,荧光分光光度法测细胞释放一氧化氮(NO)量及细胞内脂质过氧化物丙二醛(MDA)的生成量,分光光度法测细胞乳酸脱氢酶(LDH)释放量。结果:10~(-2)、10~(-3)、10~(-4)、10~(-5)mg/ml槲皮素可促进高糖损伤的VECs增殖,10~(-2)、10~(-3) mg/ml槲皮素,可抑制高糖损伤的VECs释放LDH,减少MDA生成量,促进其释放NO。结论:槲皮素对高糖损伤VECs有保护作用。

Protection of Vascllar Endothelial Cells from High Glucose Injury Induced by Quercetin

Objective:To evaluate the effects of quercetin on the high glucose-injured vascular endothelial cells (VECs) .Methods:Experiments were divided into control group, injured group and quercetin group. The cultured VECs were injured by high glucose. The proliferation of VECs was assessed by MTT assay.The amount of NO and lipid peroxidation (monitored as malonaldehyde,MDA) of VECs were assessed by fluorometric assay.The amount of lactate dehydrogenase (LDH) was assessed by spectrophotometric method.Results: 10-2 , 10-3 , 10-4 ,10-5 mg/ml quercetin increased the proliferation of high glucose-injured VECs. 10-2,10-3mg/ml quercetin reduced LDH release and MDA production, increased NO release.Conclusion:Quercetin can protect cultured VECs from being injured by high glucose.