TUNEL法检测ER(-)乳癌细胞凋亡和Ki-67表达的研究 |
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引用本文: | 刘大川,姚榛祥. TUNEL法检测ER(-)乳癌细胞凋亡和Ki-67表达的研究[J]. 重庆医科大学学报, 2001, 26(2): 151-153 |
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作者姓名: | 刘大川 姚榛祥 |
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作者单位: | 重庆医科大学临床学院普外科 |
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摘 要: | 目的:探讨雌激素受体阴性乳癌细胞中Ki-67与细胞凋亡之间的关系。方法:通过1.25(OH)2D3诱发雌激素受体阴性乳癌细胞株MDA-MB-435s凋亡,采用MTT法检测细胞增殖情况,TUNEL法检测细胞凋亡指数,免疫组化法检测Ki-67阳性表达指数,进行相关性分析。结果:在10^-8mmol/L 1.25(OH)2D3诱发MDA-MB-435s凋亡过程中,肿瘤细胞 凋亡指数与Ki-67阳性表达指数呈负相关。结论:对凋亡指数及Ki-67阳性表达指数的综合分析,可全面反映细胞凋亡和增殖的平衡状态,对于判断肿瘤细胞的增生具有参考价值。
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关 键 词: | 1 25(OH)2D3 雌激素受体 阴性 MDA-MB-435s 凋亡 Ki-67 乳腺癌 TUNEL法 细胞 |
文章编号: | 0253-3626(2001)02-0151-03 |
修稿时间: | 2000-10-20 |
Study of the expression of Ki-67 and apoptosis tested by TUNEL in the ER(-) breast cancer cell line |
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Abstract: | Objective: To test the relationship between Ki-67 index and apoptosis in the ER(-) breast cancer cell line. Methods: Apoptosis was induced by 1,25(OH)2D3 in the ER(-) breast cancer cell line. We compared the cell number by MTT, determined quantitatively apoptosis cells by TUNEL, and detected the expression of Ki-67 index immunohistochemically. Results: The expression of the Ki-67 index was decreased during apoptosis that induced by 10-8mmol/L 1,25(OH)2D3 in MDA-MB-435s cell line. there was negative correlation between the apoptosis and the expression of Ki-67 index. Conclusion: A comprehensive analysis of the apoptosis and Ki-67 index reflected the balance of proliferation and apoptosis. |
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