缺血再灌注对大鼠翼腭神经节一氧化氮合酶阳性神经元数目的影响

目的：观察缺血再灌注后大鼠翼腭神经一氧化氮合酶（NOS）阳性神经元数目的变化。方法：结扎大鼠双侧颈总动脉不同时间（15、30、60、120min)后，应用还原型尼克酰胺腺嘌呤二核苷酸磷酸－黄递酶（NADPH－d）组织化学染色法观察并计数翼腭神经节内NOS阳性神经元，结果：缺血30min组，翼腭神经节内NOS阳性神经元数目明显增加，60min组与30min组相比未见明显差异，120min组则比其它组都显著增加。结论：在缺血再灌注过程中，翼腭神经节内NOS阳性神经元数目，可能影响到脑血管周围NOS阳性纤维的NO释放量。

The Effects of Ischemia and Reperfusion on the Quantity of Nitric Oxide Synthase Positive Cells Of the Pterygopalatine Ganglion in Rats

Objective:To observe the quantitative variation of nitric oxide sythase (NOS) positive cells of pterygopalatine ganglion in rat after ischemia and reperfusion. Methods:The NOS positive cells in the pterygopalatine ganglion were quantitated by histochemistry reduced nicotinamide adenine dinucleotid phoshate diaphorase (NADPH d) staining after different ischemic periods (15, 30, 60, 120 min) by bilaterally ligated common carotid artery. Results:The number of the NOS positive neurons of pterygopalatine ganglion was greatly upregulated in the 30 min group and there was not obvious difference in both 30 min and 60 min groups, however, the number of NOS positive cells in 120 min group significantly increased compared with any other groups. Conclusion:The number of NOS positive cell of the pterygopalatine ganglion upsurged and might affect the released quantity of nitric oxide which was produced in NOS positive nerve fibers around the cerebral artery during the period of the ischemia and reperfusion.