慢性应激对大鼠海马区5-HT2AR及其mRNA表达的影响

目的：探讨慢性应激对大鼠海马区5-HT2A受体及其mRNA表达的影响。方法：选择open-feild法评分相近的20只成年SD大鼠随机分为对照组和模型组。应用分养和长期不可预见的中等强度应激造成大鼠抑郁模型，以免疫组化法和原位杂交法检测大鼠海马5-HT2AR及其mRNA的表达。结果：（1）模型组体重增长14d时明显低于对照组。（2）模型组水平活动和垂直活动于第7天后明显降低；糖水消耗量于第14天后明显减少。（3）模型组海马5-HT2AR及其mRNA表达均低于对照组（均P〈0．01）。结论：慢性应激能诱发大鼠轻度抑郁，其机制与海马5-HT2AR及其mRNA的低表达有关。

The Effect of Chronic Mild Stress on Hippocampus 5 - HT2AR and 5 -HT2ARmRNA in Rats

Objective： To investigate the effect of chronic mild stress on hippocampus 5 - HT2AR and 5 - HT2ARmRNA in rats, Methods： 20 Sprague- Dawley rats were randomly divided into 2 groups, The models animals were given separation and chronic unpredictable mild stress. Immunohistochemistry and hybridization in situ were used to study the changes of 5 - HT2AR and 5 - HT2A- RmRNA in the hippocampus of rats. Results： （1） The body weight gains on the fourteenth stressed days. （2） The level score and vertical score were lower after seven days in model group and the saeehar consumption were lower after fourteen days in model group, （3） The 5 -HT2AR and 5 - HT2AR mRNA express were lower in model group than in control（P 〈 0.01）. Conclusion： The results indicate that the chronic unpredictable mild stress may induce the mild depression and decrease the 5 - HT2AR and 5 - HT2AR mR- NA levd in hippocampus.