| 河南不同产地夏枯草中迷迭香酸的对比分析 |
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| 引用本文： | 陈志红.河南不同产地夏枯草中迷迭香酸的对比分析[J].河南中医药学刊,2008(4):36-37. |
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| 作者姓名： | 陈志红 |
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| 作者单位： | 河南中医学院分析测试中心,河南郑州450008 |
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| 基金项目： | 河南省教育厅科学研究项目（编号：2003350178） |
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| 摘 要： | 目的：测定河南不同产地夏枯草中迷迭香酸的含量以比较夏枯草质量的优劣，为夏枯草的质量控制方法提供一定的依据。方法：采用高效液相法测定迷迭香酸色谱条件为：采用Betasil C18（250mm×4．6mm，5μm）；流动相：甲醇-1％甲酸（40：60）流速：1．0ml／min；柱温：25℃；检测波长：330nm；结果：迷迭香酸在0．44-8．80μg范围内线性关系良好；r=0．99999；平均回收率为98．17％，RSD％为1．02％。结论：该方法简单、准确并且专属性强。
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| 关 键 词： | 夏枯草 迷迭香酸 质量分析 |
The Content Determination of Rosmarinic Acid of Defferent Areas of Henan＇s Spica Prunellae |
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| Chen Zhihong.The Content Determination of Rosmarinic Acid of Defferent Areas of Henan＇s Spica Prunellae[J].Journal of Henan college of Traditional Chinese Medicine,2008(4):36-37. |
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| Authors: | Chen Zhihong |
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| Institution: | Chen Zhihong(Center of Analysis and Measurement of Henan College of TCM, Zhengzhou, Henan, Chian, 450008) |
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| Abstract: | Objective： To establish a HPLC method for content determination of rosmarinic acid in different area Spiea Prunellae Method： The rosmarinic acid separation was performed on a Betasil G8 （250 mm × 4.6 mm,5 μm）column, the mobile phase was composed of methanol- 1% formic acid（40：60）, the flow rate was 1.0 ml/min, the ultraviolet wavelength was 330 nm, the column temperature was 2512. Result： The rosmarinic acid calibration curve showed good linearity in the range of 0.44-8.80 μg（r= 0. 9999）, the average recovery was 98.17% （RSD= 1.02% ）. Conclusion. The method is simple exact, accurate and exclusive. |
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| Keywords: | Spica Prunellae rosmarinic acid quality analysis |
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