| Expression of Vascular Endothelial Growth Factor （VEGF） in Human Osteosarcoma Cells Transfected with Adeno-associated Virus-antisense VEGF |
| |
| 引用本文： | 徐卫国,陈安民,张衣北,易成腊. Expression of Vascular Endothelial Growth Factor （VEGF） in Human Osteosarcoma Cells Transfected with Adeno-associated Virus-antisense VEGF[J]. 华中科技大学学报(医学英德文版), 2004, 24(3): 279-281. DOI: 10.1007/BF02832012 |
| |
| 作者姓名： | 徐卫国 陈安民 张衣北 易成腊 |
| |
| 作者单位： | Department of Orthopaedics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Orthopaedics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Orthopaedics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China，Department of Orthopaedics，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China |
| |
| 摘 要： | Ithasbecomeacommonmethodforcancertreatmentthroughantivascularizationinthepresent.Somestudieshaverevealedthatlivercancer ,lungcancerandcervicalcancercanbetreatedsuccessfullythroughholdingbackvascularendothelialgrowthfac tor (VEGF) proteinbecauseofitsimport…
|
| 关 键 词： | 血管内皮生长因子 VEGF 骨肉瘤细胞 病毒转染 腺样增殖作用 基因表达 |
| 收稿时间： | 2003-12-23 |
Expression of vascular endothelial growth factor (VEGF) in human osteosarcoma cells transfected with adeno-associated virus-antisense VEGF |
| |
| Xu Weiguo,Chen Anmin,Zhang Yibei,YI Chengla. Expression of vascular endothelial growth factor (VEGF) in human osteosarcoma cells transfected with adeno-associated virus-antisense VEGF[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2004, 24(3): 279-281. DOI: 10.1007/BF02832012 |
| |
| Authors: | Xu Weiguo Chen Anmin Zhang Yibei YI Chengla |
| |
| Affiliation: | Department of Orthopaedics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| |
| Abstract: | Summary The expression of protein vascular endothelial growth factor (VEGF) in osteosarcoma cells transfected with adeno-associated virus (rAAV)-antisense VEGF was studied to provide the foundation of osteosarcoma treatment through antivascularization. The rAAV-antisense VEGF at different doses (0, 20, 50, 100, 200, 240 μl) was transfected into osteosarcoma MG-63 cell. The cells and culture supernatants were collected before and after tansfection. The expression of VEGF protein was detected by using immunohistochemical staining (SP) and Western blot. SP and Western-blot tests revealed that the MG-63 Cells transfected with rAAV-antisense VEGF had less staining than those without transfection with rAAV-antisense VEGF, and the staining intensity was negatively correlated with the doses of genes. The corresponding A values of transfected genes with different doses of rAAV-antisense VEGF (0, 20, 50, 100, 200, 240 μl) were 86 614±13 776, 73 245±15 414. 61 078±12 124, 54 657±10 953, 39 802±11 308, 32 014±15 057 respectively, with the difference being significant (P<0.05). It was concluded that the expression of VEGF protein in MG-63 cells could be inhibited by rAAV-antisense VEGF. XU Weiguo, male, born in 1970, M. D., Ph. D. |
| |
| Keywords: | adeno-associated virus antisense vascular endothelial growth factor gene transfection osteosarcoma |
| 本文献已被 CNKI 万方数据 SpringerLink 等数据库收录！ |
|
