不同治法体内抗甲3(H3N2)亚型流感病毒作用的实验研究

目的:探讨辛凉解表、辛温解表及祛湿解表等不同治法在体内抗甲3(H3N2)亚型流感病毒的作用。方法:三种治法的代表方分别选择银翘散、桂枝麻黄各半汤和新加香薷饮,设正常对照组、阳性药物(病毒唑)组和三种治法组,分别观察不同治法对甲3(H3N2)亚型流感病毒小鼠肺炎的影响,对小鼠感染甲3(H3N2)亚型流感病毒的死亡保护作用和延长生命作用。结果:以肺指数为评价指标,三种不同治法组与模型对照组相比(P<0.01),差异有统计学意义;三种不同治法组之间比较以及三种不同治法组和阳性药物组之间比较,差异无统计学意义。不同处理组间死亡率比较和不同处理组间存活时间的比较,差异无统计学意义。结论:三种不同治法对甲3(H3N2)亚型流感病毒小鼠肺炎均有抑制作用,但三种不同治法之间以及三种不同治法和阳性药物之间作用并无差异性。三种治法对小鼠感染甲3(H3N2)亚型流感病毒的死亡保护作用和延长生命作用均不明显。

Experimental Research on the Effects of Different Diaphoretic Therapeutic Methods Against Influenza A (H3N2) Viruses in Vivo

Objective: To explore the effects of different diaphoretic therapeutic principles(acrid and hot-natured drugs,acrid and cool-natured drugs and moisture-eliminating drugs) against influenza A(H_3N_2) viruses in vivo.Methods: Yinqiao Powder,Guima Geban Decoction and Xinjia Xiangru Decoction were chosen to represent different diaphoretic therapeutic methods. NIH mice were divided randomly into the controlled group and the experimental group.The Experimental group included three decoction-treated mice and Ribavirin-treated mice.To observe the effects of three different principles on treating mice influenza A(H_3N_2) pneumonia,decreasing mortality of infected mice and prolonging the survival of mice infected with influenza A(H_3N_2) viruses.Results: There were significant differences in the score of lung consolidation between decoction-treated mice and virus-infected mice(P<0.01).But there were no significant differences in SLC among three different decoction-treated mice and between three different decoction-treated mice and Ribavirin-treated mice.Meanwhile the experiment also showed there were no significant differences in mice mortality and survival among respective groups.Conclusion: Three different principles all can relieve the seriousness of mice influenza A (H_3N_2) pneumonia.But the effects of three different methods on mortality of infected mice and prolonging the survival of infected mice are not remarkable.